Liquid crystal precursor system as a vehicle for curcumin-mediated photodynamic inactivation of oral biofilms

Autor(es)

Reina, Barbara Donadon ; Santezi, Carolina ; Malheiros, Samuel Santana ; Calixto, Giovana ; Rodero, Camila ; Victorelli, Francesca Damiani ; Chorilli, Marlus ; Dovigo, Livia Nordi

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. N/A, p. 12-pg., 2022-10-13.

Resumo

Curcumin has great potential as a photosensitizer, but it has low solubility in aqueous solutions. This study reports the antimicrobial efficacy of photodynamic inactivation (PDI) mediated by a curcumin-loaded liquid crystal precursor (LCP) on in situ dental biofilms. Thirty volunteers used intraoral devices containing enamel samples for 48 hours for biofilm formation. The samples were then removed from the device and treated either with LCP with 160 mu M of curcumin plus illumination at 18 J/cm(2) (C + L+ group) or with LCP without curcumin in the dark (C - L - group). Following this, the biofilm from the samples was plated for quantifying the viable colonies at 37 degrees C for 48 hours. Specific and nonspecific media were used for the presumptive isolation of Streptococcus mutans, Lactobacillus species/aciduric microorganisms, Candida species, and total microbiota. The C + L+ group showed a highly significant (P < .001) reduction in the log(10) (colony forming units/mL) values as compared to the C - L - group for all culture media. Hierarchical linear regression indicated that there may be predictors at individual volunteer level explaining the difference in the PDI efficacy among different individuals (P = .001). The LCP system retained curcumin and released it slowly and continuously, thus protecting the drug from photodegradation. LCP with curcumin is considered effective for the photoinactivation of dental biofilms, but the PDI efficacy may differ based on the host's individual characteristics. (AU)

• Processo FAPESP: 16/24918-5 - Eficácia da inativação fotodinâmica mediada pela curcumina sobre biofilme dental formado in situ
• Beneficiário: Bárbara Donadon Reina
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/24112-8 - Eficácia da Inativação Fotodinâmica mediada pela Curcumina sobre biofilme dental formado in situ
• Beneficiário: Lívia Nordi Dovigo
• Modalidade de apoio: Auxílio à Pesquisa - Regular