Detection of intestinal parasites in human faecal samples using dissolved air flotation

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Soares, Felipe Augusto ; Dos Santos, Bianca Martins ; Rosa, Stefany Laryssa ; Nery Loiola, Saulo Hudson ; Stefano, Vitoria Castilho ; Proenca, Leticia Rodrigues ; Nagase Suzuki, Celso Tetsuo ; Sabadini, Edvaldo ; Saraiva Bresciani, Katia Denise ; Falcao, Alexandre Xavier ; Gomes, Jancarlo Ferreira

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL MEDICINE & INTERNATIONAL HEALTH; v. 27, n. 12, p. 9-pg., 2022-11-01.

Resumo

Objective Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF. Methods In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF-Test) for triplicate DAF tests. We evaluated physical-chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non-floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results. Results The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively. Conclusion The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection. (AU)

• Processo FAPESP: 14/12236-1 - AnImaLS: Anotação de Imagem em Larga Escala: o que máquinas e especialistas podem aprender interagindo?
• Beneficiário: Alexandre Xavier Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/50501-4 - Estudo e viabilização de novos paradigmas de concentração e identificação parasitária
• Beneficiário: Jancarlo Ferreira Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 22/04349-7 - Processamento de amostras fecais por meio da integração da flotação por ar dissolvido e diagnóstico automatizado de parasitos intestinais
• Beneficiário: Felipe Augusto Soares
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado