Vitamins Modulate the Expression of Antioxidant Genes in Progesterone-Treated Pancreatic beta Cells: Perspectives for Gestational Diabetes Management

Autor(es)

Borcari, Nathalia Ruder ; dos Santos, Jeniffer Farias ; Reigado, Gustavo Roncoli ; Freitas, Bruna Leticia ; Araujo, Mariana da Silva ; Nunes, Viviane Abreu

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ENDOCRINOLOGY; v. 2020, p. 14-pg., 2020-09-15.

Resumo

Gestational diabetes (GD) is a condition defined as carbohydrate intolerance and hyperglycemia beginning in the second trimester of pregnancy, which overlaps with the progesterone exponential increase. Progesterone has been shown to cause pancreatic beta-cell death by a mechanism dependent on the generation of reactive oxygen species and oxidative stress. Herein, we studied the effect of this hormone on the expression of 84 genes related to oxidative stress and oxidant defense in pancreatic RINm5F cell lineage. Cells were incubated with 0.1, 1.0, or 100 mu M progesterone for 6 or 24 h, in the presence or absence of the vitamins E and C. Among the investigated genes, five of them had their expression increased, at least 2-fold, in two different concentrations independently of the time of incubation, or at the same concentration at the different time points, including those that encode for stearoyl-CoA desaturase 1 (Scd1), dual oxidase 1 (Duox1), glutathione peroxidase 6 (GPx6), heme oxygenase 1 (Hmox1), and heat shock protein a1a (Hspa1a). Vitamins E and C were able to increase, in progesterone-treated cells, the expression of genes with antioxidant function such asHmox1, but decreasedScd1expression, a gene with prooxidant function. At cytoplasmic level, progesterone positively modulatedHmox1andHspa1acontent. These results suggest that the protein encoded by these genes might protect cells against progesterone induced-oxidative damage, opening perspectives to elucidate the molecular mechanism involved in progesterone action in GD, as well as for the development of antioxidant strategies for the prevention and treatment of this disease. (AU)

• Processo FAPESP: 16/14150-2 - Efeito da progesterona na expressão de genes envolvidos no estresse oxidativo e defesa antioxidante em células beta pancreáticas e a relação entre o estado redox no plasma de gestantes e desenvolvimento do diabetes gestacional
• Beneficiário: Viviane Abreu Nunes Cerqueira Dantas
• Modalidade de apoio: Auxílio à Pesquisa - Regular