| Texto completo | |
| Autor(es): |
Cury, S. S.
;
Oliveira, J. S.
;
Biagi-Junior, C. A. O.
;
Silva Jr, W. A.
;
Reis, P. P.
;
Cabral-Marques, O.
;
Hasimoto, E. N.
;
Freire, P. P.
;
Carvalho, R. F.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Gene; v. 852, p. 11-pg., 2023-02-05. |
| Resumo | |
Lung cancer patients with COVID-19 present an increased risk of developing severe disease and, consequently, have poor outcomes. Determining SARS-CoV-2-host interactome in lung cancer cells and tissues, infected or uninfected with SARS-CoV-2, may reveal molecular mechanisms associated with COVID-19 development and severity in lung cancer patients. Here, we integrated transcriptome data of lung tumors from patients with small -or non-small cell lung cancer (SCLC and NSCLC) and normal lung and lung cancer cells infected with SARS-CoV-2. We aimed to characterize molecular mechanisms potentially associated with COVID-19 development and severity in lung cancer patients and to predict the SARS-CoV-2-host cell interactome. We found that the gene expression profiles of lung cell lines infected with SARS-CoV-2 resemble more primary lung tumors than non-malignant lung tissues. In addition, the transcriptomic-based interactome analysis of SCLC and NSCLC revealed increased expression of cancer genes BRCA1 and CENPF, whose proteins are known or predicted to interact with the SARS-CoV-2 spike glycoprotein and helicase, respectively. We also found that TRIB3, a gene coding a putative host-SARS-CoV-2 interacting protein associated with COVID-19 infection, is co-expressed with the up-regulated genes MTHFD2, ADM2, and GPT2 in all tested conditions. Our analysis identified biological processes such as amino acid metabolism and angiogenesis and 22 host mediators of SARS-CoV-2 infection and replication that may contribute to the development and severity of COVID-19 in lung cancers. (AU) | |
| Processo FAPESP: | 13/50343-1 - Genomic profiling of messenger RNAs and microRNAs in cancer cachexia |
| Beneficiário: | Robson Francisco Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/09146-1 - Análise sistêmica e integrativa dos mecanismos moleculares associados à imunorregulação em pacientes com COVID-19 |
| Beneficiário: | Paula Paccielli Freire |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |