STAT3 signaling modulates the immune response induced after antigen targeting to conventional type 1 dendritic cells through the DEC205 receptor

Autor(es)

Sulczewski, Fernando Bandeira ; Martino, Larissa Alves ; Salles, Davi ; Yamamoto, Marcio Massao ; Rosa, Daniela Santoro ; Boscardin, Silvia Beatriz

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 13, p. 15-pg., 2022-10-18.

Resumo

Conventional dendritic cells (cDC) are a group of antigen-presenting cells specialized in priming T cell responses. In mice, splenic cDC are divided into conventional type 1 DC (cDC1) and conventional type 2 (cDC2). cDC1 are specialized to prime the Th1 CD4(+) T cell response, while cDC2 are mainly associated with the induction of follicular helper T cell responses to support germinal center formation. However, the mechanisms that control the functions of cDC1 and cDC2 are not fully understood, especially the signaling pathways that can modulate their ability to promote different CD4(+) T cell responses. Here, we targeted a model antigen for cDC1 and cDC2, through DEC205 and DCIR2 receptors, respectively, to study the role of the STAT3 signaling pathway in the ability of these cells to prime CD4(+) T cells. Our results show that, in the absence of the STAT3 signaling pathway, antigen targeting to cDC2 induced similar frequencies of Tfh cells between STAT3-deficient mice compared to fully competent mice. On the other hand, Th1 and Th1-like Tfh cell responses were significantly reduced in STAT3-deficient mice after antigen targeting to cDC1 via the DEC205 receptor. In summary, our results indicate that STAT3 signaling does not control the ability of cDC2 to promote Tfh cell responses after antigen targeting via the DCIR2 receptor, but modulates the function of cDC1 to promote Th1 and Th1-like Tfh T cell responses after antigen targeting via the DEC205 receptor. (AU)

• Processo FAPESP: 18/20821-2 - Análise da influência da interleucina 10 nas repostas celular e humoral promovida pelo direcionamento de antígenos para células dendríticas CD8a+DEC205+
• Beneficiário: Larissa Alves Martino
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/00145-2 - Influência das vias de sinalização de STAT1, STAT3, STAT5 e STAT6 em células dendríticas convencionais na instrução da resposta de células T auxiliares
• Beneficiário: Fernando Bandeira Sulczewski
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50890-5 - INCT 2014: Investigação em Imunologia
• Beneficiário: Jorge Elias Kalil Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/17471-7 - Antigenicidade e imunogenicidade de proteínas recombinantes do envelope viral do Zika vírus
• Beneficiário: Daniela Santoro Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/07142-9 - Influência das Vias de Sinalização de STAT1, STAT3, STAT5 e STAT6 em Células Dendríticas Convencionais na Instrução da Resposta de Células T Auxiliares
• Beneficiário: Silvia Beatriz Boscardin
• Modalidade de apoio: Auxílio à Pesquisa - Regular