Production, Physicochemical and Structural Characterization of a Bioemulsifier Produced in a Culture Medium Composed of Sugarcane Bagasse Hemicellulosic Hydrolysate and Soybean Oil in the Context of Biorefineries

Autor(es)

Barbosa, Fernanda Goncalves ; Marcelino, Paulo Ricardo Franco ; Lacerda, Talita Martins ; Philippini, Rafael Rodrigues ; Giancaterino, Emma Teresa ; Mancebo, Marcos Campos ; dos Santos, Julio Cesar ; da Silva, Silvio Silverio

• Tipo de documento: Artigo Científico
• Fonte: FERMENTATION-BASEL; v. 8, n. 11, p. 21-pg., 2022-11-01.

Resumo

Biosurfactants are amphipathic molecules, biodegradable, with reduced toxicity. They can be synthesized by fermentative processes from oleaginous compounds and agro-industrial by-products. In this context, the present study describes the production and the physical, chemical, and structural characterization of the bioemulsifier secreted by the yeast Scheffersomyces shehatae 16-BR6-2AI in a medium containing hemicellulosic sugarcane bagasse hydrolysate combined with soybean oil. The bioemulsifier was produced in Erlenmeyer flasks and isolated; then, the physicochemical and structural characterization of the formed molecule was carried out. The following fermentation parameters were obtained: Y-X/S = 0.45, Y-P/S = 0.083, and productivity of 0.076 g/L/h. The bioemulsifier was found to be a polymer containing 53% of carbohydrates, 40.92% of proteins, and 6.08% of lipids, respectively. The FTIR spectrum confirmed the presence of functional groups such as amides, amines, and carbonyls. The bioemulsifier was stable over a range of temperature (-20 degrees C to 120 degrees C), salinity (1-15%), and pH (2-12). It was observed that the biomolecule has a better emulsifying action in organic solvents with a non-polar character. Therefore, this biomolecule is a potential substitute for synthetic surfactants and can be used in different applications. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 16/14852-7 - Uma solução sustentável contra doenças tropicais negligenciadas utilizando biossurfactantes produzidos por leveduras em hidrolisado hemicelulósico de bagaço de cana-de-açúcar
• Beneficiário: Paulo Ricardo Franco Marcelino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado