High-fat diet decreases H3K27ac in mice adipose-derived stromal cells

Autor(es)

da Silva, Viviane S. ; Simao, Jussara J. ; Plata, Victor ; de Sousa, Andressa Franca ; da Cunha de Sa, Roberta D. C. ; Machado, Cleiton Fagundes ; Stumpp, Taiza ; Alonso-Vale, Maria Isabel C. ; Armelin-Correa, Lucia

• Tipo de documento: Artigo Científico
• Fonte: OBESITY; v. 30, n. 10, p. 10-pg., 2022-09-05.

Resumo

Objective The study goal was to analyze the effects of a high-fat diet (HFD) on the histone 3 lysine 27 (H3K27) posttranscriptional modifications and the expression of histone-modifying enzymes in adipose-derived stromal cells (ASCs) from white adipose tissue (WAT). Methods Male C57BL/6J mice received control or HFD for 12 weeks. The ASCs were isolated from subcutaneous and visceral (epididymal) WAT, cultivated, and evaluated for expression of H3K27 trimethylation (H3K27me3) and H3K27 acetylation (H3K27ac) by Western blot. The transcription of histone-modifying enzymes was analyzed by real-time polymerase chain reaction. Results When compared with control, HFD ASCs showed a decrease in H3K27ac enrichment in subcutaneous and visceral WAT and ATP-citrate lyase expression in subcutaneous WAT. Curiously, the expression of CREB-binding protein was increased in visceral ASCs from HFD-fed mice. Conclusions These results show that an HFD significantly reduces acetylation of H3K27 in ASCs and the expression of ATP-citrate lyase in subcutaneous ASCs, suggesting that, in this fat depot, the H3K27ac reduction could be partly due to lower acetyl-coenzyme A availability. H3K27ac is an epigenetic mark responsible for increasing the transcription rate and its reduction can have an important impact on ASC proliferation and differentiation potential. (AU)

• Processo FAPESP: 19/13618-9 - Estudo da modulação da expressão de Ezh2 e de outros modificadores de H3K27 por fatores da transcrição NF-kB em células adiposas
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/26240-4 - Vias de sinalização afetadas pelo óleo de peixe em células tronco derivadas do tecido adiposo (AdSCs) e adipócitos: correlação com alterações metabólicas, endócrinas e na adipogênese que repercutem em seus efeitos anti-Obesidade Hipertrófica
• Beneficiário: Jussara de Jesus Simão
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/05485-6 - Efeitos da dieta hiperlipídica e da suplementação com ácidos graxos ômega-7 e ômega-3 sobre o processo de browning, proliferação e diferenciação de células mesenquimais contidas na fração celular estromal do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular