| Texto completo | |
| Autor(es): |
Ribeiro, Arthur Barcelos
;
Nicolella, Heloiza Diniz
;
Domingos da Silva, Lucas Henrique
;
Aldana Mejia, Jennyfer Andrea
;
Tanimoto, Matheus Hikaru
;
Ambrosio, Sergio Ricardo
;
Bastos, Jairo Kenupp
;
Orenha, Renato Pereira
;
Tame Parreira, Renato Luis
;
Tavares, Denise Crispim
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Planta Medica; v. N/A, p. 10-pg., 2022-09-28. |
| Resumo | |
Guttiferone E (GE) is a benzophenone found in Brazilian red propolis. In the present study, the effect of GE on human (A375) and murine (B16-F10) melanoma cells was investigated. GE significantly reduced the cellular viability of melanoma cells in a time-dependent manner. In addition, GE demonstrated antiproliferative effect, with IC50 values equivalent to 9.0 and 6.6 mu M for A-375 and B16-F10 cells, respectively. The treatment of A-375 cells with GE significantly increased cell populations in G0/G1 phase and decreased those in G2/M phase. Conversely, on B16-F10 cells, GE led to a significant decrease in the populations of cells in G0/G1 phase and concomitantly an increase in the population of cells in phase S. A significantly higher percentage of apoptotic cells was observed in A-375 (43.5%) and B16-F10 (49.9%) cultures after treatment with GE. Treatments with GE caused morphological changes and significant decrease to the melanoma cells' density. GE (10 mu M) inhibited the migration of melanoma cells, with a higher rate of inhibition in B16-F10 cells (73.4%) observed. In addition, GE significantly reduced the adhesion of A375 cells, but showed no effect on B16-F10. Treatment with GE did not induce changes in P53 levels in A375 cultures. Molecular docking calculations showed that GE is stable in the active sites of the tubulin dimer with a similar energy to taxol chemotherapy. Taken together, the data suggest that GE has promising antineoplastic potential against melanoma. (AU) | |
| Processo FAPESP: | 18/25770-7 - Estudos in vitro, in vivo e in silico da atividade antineoplásica da gutiferona e em melanoma |
| Beneficiário: | Arthur Barcelos Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |