Acquired Pellicle Engineering Using a Combination of Organic (Sugarcane Cystatin) and Inorganic (Sodium Fluoride) Components against Dental Erosion

Autor(es)

Pela, Vinicius Taioqui ; Niemeyer, Samira Helena ; Baumann, Tommy ; Levy, Flavia Mauad ; Henrique-Silva, Flavio ; Lussi, Adrian ; Carvalho, Thiago Saads ; Rabelo Buzalaf, Marilia Afonso

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 56, n. 2, p. 8-pg., 2022-06-01.

Resumo

This study evaluated the combination of a sugarcane cystatin (CaneCPl-5) and sodium fluoride (NaF) in acquired pellicle engineering for the prevention of dental erosion in vitro. Seventy-five human enamel specimens were prepared and divided into 5 treatment groups (n = 15/group): Deionized water (Control); Elmex (TM) (SnCl2/NaF/AmF); 0.1 mg/mL Ca neCPI-5; 500 ppm NaF; and CaneCPl-5+NaF (Combination). The specimens were individually treated (200 mu L; 2 min; 37 degrees C), then incubated in human saliva (200 mu L; 1 h, at 37 degrees C) for acquired pellicle formation. Afterward, the specimens were submitted to an erosive challenge (1% citric acid [CRI, pH 3.6, 10 mL, 2 min, 25 degrees C). This sequence was conducted S times. Percentage of surface microhardness change (%SMC), relative surface reflection intensity (rSRI), and calcium released to the CR were measured and analyzed by one-way ANOVA followed by Tukey's test (p < 0.05). In general, all the treatments (SnCl2/NaF/AmF, CaneCPl-5, NaF, and Combination) significantly protected the enamel when compared the control group. Regarding %SMC and rSRI, the Combination was the most effective treatment, reducing the %SMC significantly (p < 0.01) when compared to all the other treatments, although this difference was not significant in the CR analysis. All treatments demonstrated a protective effect on enamel against dental erosion; however, the combination of CaneCPl-5 with NaF showed a greater protection. (C) 2022 S. Karger AG, Basel (AU)

• Processo FAPESP: 19/08437-5 - Engenharia da película adquirida para proteção contra a erosão dentária: uso de um novo refletômetro óptico portátil para avaliar o papel protetor de proteínas/peptídeos
• Beneficiário: Vinícius Taioqui Pelá
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado