Probiotic consumption can modify the resilience of periodontal tissues in rats under experimental periodontitis

Autor(es)

de Oliveira, Luiz Fernando Ferreira ; Silva, Pedro Henrique Felix ; Salvador, Sergio Luiz ; Ervolino, Edilson ; Casarin, Renato ; Figueiredo, Luciene ; Ricoldi, Milla Tavares ; de Souza, Sergio Luis Scombatti ; Furlaneto, Flavia ; Messora, Michel Reis

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 94, n. 2, p. 13-pg., 2022-12-27.

Resumo

BackgroundThis study evaluated the effects of systemic administration of Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) on experimental periodontitis (EP) in rats. MethodsThirty-two rats were allocated to groups C (control), C-HN019 (probiotic), EP (EP only), and EP-HN019 (EP+probiotic). From day 0, the animals of C-HN019 and EP-HN019 groups received B. lactis HN019 (1 x 10(9) CFU/ml) daily. On the 14th day, the animals of EP and EP-HN019 groups received silk ligature around mandibular molars. Animals were euthanized on the 28th day. Samples of oral biofilm, gingival tissues, blood serum, and mandible were obtained for microtomographic, histomorphometric, microbiological, and immunological analyses. Data were statistically analyzed (p < 0.05). ResultsGroup EP-HN019 presented significantly less alveolar bone loss when compared with Group EP in histomorphometric and microtomographic analyses. In gingival tissue and serum, Group EP-HN019 presented lower proinflammatory/anti-inflammatory cytokines ratios than Group EP. Group EP-HN019 showed higher expression of beta-defensins and less TRAP-positive cells than Group EP. Group EP presented higher gene expression of Ifng and lower gene expression of Foxp3 when compared with Group EP-HN019 in gingival tissue. In oral biofilm, EP-HN019 group presented a lower percentage of species similar to Fusobacterium periodonticum and a higher percentage of species similar to Actinomyces gereneseriae, Actinomyces israelli, and Streptococcus gordonii when compared with Group EP. There was a significant increase of B. lactis HN019 after administration of probiotic therapy in oral biofilm of Group EP-HN019. ConclusionThe consumption of B. lactis HN019 promotes a protective effect against alveolar bone loss by modifying local and systemic microbiological and immunoinflammatory parameters. (AU)

• Processo FAPESP: 13/25022-7 - Efeitos da terapia com Bifidobacterium animalis subsp. lactis HN019 e Bdellovibrios bacteriovorus HD100 na periodontite experimental em ratos: estudo do perfil microbiológico, imunológico, histológico e genético da resposta do hospedeiro
• Beneficiário: Michel Reis Messora
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11835-1 - Efeitos da terapia com Bifidobacterium animalis subsp. lactis HN019 e Bdellovibrios bacteriovorus HD100 na periodontite experimental em ratos: estudo do perfil microbiológico, imunológico, histológico e genético da resposta do hospedeiro
• Beneficiário: Pedro Henrique Felix Silva
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico