Expression of DNA repair in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction

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Oliveira Alves, Monica Ghislaine ; Miguel, Natalia da Silva ; Panisello Ferreira, Camila Cristina ; Alvarenga, Elis Ribeiro ; Tonon, Bruna Manzanares ; de Barros, Patricia Pimentel ; Lima Carta, Celina Faig ; Nogueira Paschoal, Maria Beatriz ; de Sales Chagas, Jose Francisco ; Bandeira, Celso Muller ; Nunes, Fabio Daumas ; Almeida, Janete Dias

• Tipo de documento: Artigo Científico
• Fonte: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 130, n. 3, p. 8-pg., 2020-09-01.

Resumo

Objective. The aim of this study was to evaluate the expression of DNA repair genes in cases of oral squamous cell carcinoma (OSCC). Study Design. Expression of the MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was evaluated by reverse transcription-quantitative polymerase chain reaction in the OSCC group (32 patients) and the control group (15 patients). The groups were compared by using the Mann-Whitney test, with Bonferroni correction. Associations between gene expression levels and clinical data were explored by using Pearson's and Spearman's correlation coefficients, with P value less than .05 indicating a significant difference. Results. The MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes were downregulated in the OSCC group compared with the control group, with significant values for MLH1 (P < .0001); MSH2 (P = .038); MLH3 (P < .0001); ATM (P < .0001); MRE11A (P < .0001); XRCC1 (P = .0004); and PMS2 (P = .008). Analysis of the correlation between gene expression and clinical data only revealed a significant negative correlation between age and expression of the PMS2 gene. Conclusions. Expression of the DNA repair genes MLH1, MSH2, MLH3, ATM, MRE11 AMRE1 JA, XRCC1, and PMS2 was reduced in OSCC. (AU)

• Processo FAPESP: 16/08633-0 - Tabagismo: dos aspectos moleculares a abordagem do fumante
• Beneficiário: Janete Dias Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/23179-7 - Estudo da expressão de genes de MLH3 e ATM em carcinoma de células escamosas bucal
• Beneficiário: Camila Cristina Panisello Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/21227-4 - Estudo da expressão de genes de MLH1 e MLH2 em carcinoma de células escamosas bucal
• Beneficiário: Natália da Silva Miguel
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica