Vaginal Microbiome Components as Correlates of Cervical Human Papillomavirus Infection

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Pessoa Morales, Julia Andrade ; Marconi, Camila ; El-Zein, Mariam ; Ravel, Jacques ; da Silva Pinto, Gabriel Victor ; Silveira, Rosana ; de Lima, Moises Diogo ; de Carvalho, Newton Sergio ; Figueiredo Alves, Rosane Ribeiro ; Garcia de Lima Parada, Cristina Maria ; Morais Leite, Sandra Helena ; Villa, Luisa L. ; Franco, Eduardo L. ; da Silva, Marcia Guimaraes

• Tipo de documento: Artigo Científico
• Fonte: Journal of Infectious Diseases; v. 226, n. 6, p. 14-pg., 2022-01-06.

Resumo

Background Interplay between vaginal microbiome and human papillomavirus (HPV) remains unclear, partly due to heterogeneity of microbiota. Methods We used data from 546 women enrolled in a cross-sectional study in 5 Brazil. We genotyped vaginal samples for HPV and sequenced V3-V4 region of 16S rRNA gene for vaginal microbiome analysis. We used stepwise logistic regression to construct 2 linear scores to predict high-risk HPV (hrHPV) positivity: one based exclusively on presence of individual bacterial taxa (microbiome-based [MB] score) and the other exclusively on participants' sociodemographic, behavioral, and clinical (SBC) characteristics. MB score combined coefficients of 30 (of 116) species. SBC score retained 6 of 25 candidate variables. We constructed receiver operating characteristic curves for scores as hrHPV correlates and compared areas under the curve (AUC) and 95% confidence intervals (CI). Results Overall, prevalence of hrHPV was 15.8%, and 26.2% had a Lactobacillus-depleted microbiome. AUCs were 0.8022 (95% CI, .7517-.8527) for MB score and 0.7027 (95% CI, .6419-.7636) for SBC score (P = .0163). Conclusions The proposed MB score is strongly correlated with hrHPV positivity-exceeding the predictive value of behavioral variables-suggesting its potential as an indicator of infection and possible value for clinical risk stratification. This cross-sectional study demonstrated a strong correlation between vaginal microbiome components and cervical high-risk HPV positivity. (AU)

• Processo FAPESP: 12/01278-0 - Avaliação da influência da cervicite por Chlamydia trachomatis na persistência da infecção pelo papilomavírus humano (HPV) e no perfil de citocinas produzidas na secreção cervical de mulheres imunocompetentes
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/10403-2 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/18469-9 - Busca de preditores do microbioma vaginal para a infecção por papilomavírus humano (HPV) no trato genital inferior de mulheres brasileiras em idade reprodutiva
• Beneficiário: Julia Andrade Pessoa Morales
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/16800-3 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular