Busca avançada
Ano de início
Entree


The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action

Texto completo
Autor(es):
Mostrar menos -
Lazarini, Josy Goldoni ; Massarioli, Adna Prado ; Soares, Jackeline Cintra ; Nani, Bruno Dias ; Charo, Nancy ; Oliveira, Douglas Souza ; Camargo, Lauren ; Alvarez-Flores, Miryam Paola ; Correia Batista, Isabel de Fatima ; Chudzinski-Tavassi, Ana Marisa ; de Alencar, Severino Matias ; Franchin, Marcelo ; Rosalen, Pedro Luiz
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: FOOD CHEMISTRY: MOLECULAR SCIENCES; v. 4, p. 10-pg., 2022-03-09.
Resumo

We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-kappa B activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11 b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-kappa B activation, IL-1 beta, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies. (AU)

Processo FAPESP: 17/09898-0 - Bioprospecção de novas moléculas anti-inflamatórias de produtos naturais nativos brasileiros
Beneficiário:Pedro Luiz Rosalen
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/02926-6 - Fitoquímica e bioprospecção de atividades antioxidante e anti-inflamatória de frutas nativas brasileiras inexploradas.
Beneficiário:Josy Goldoni Lazarini
Modalidade de apoio: Bolsas no Brasil - Doutorado