Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows

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Juliano, L. C. B. ; Gouvea, F. L. R. ; Latosinski, G. S. ; Fabri, F. H. H. ; Salvador, T. B. ; Guimaraes, F. F. ; Ribeiro, M. G. ; Langoni, H. ; Rall, V. L. M. ; Hernandes, R. T. ; Leite, D. S. ; Pantoja, J. C. F.

• Tipo de documento: Artigo Científico
• Fonte: Letters in Applied Microbiology; v. 75, n. 4, p. 9-pg., 2022-06-27.

Resumo

The objectives were to study the diversity of Enterococcus spp. isolated from mastitis cases, milking equipment and the environment of dairy cows; and to determine in vitro resistance of isolates to antimicrobials that are relevant to human and animal health. Ten dairy farms were visited to collect samples from mastitis cases, faeces, bedding, aisles, water and milking equipment. Identification of Enterococcus at the species level and antimicrobial resistance testing was performed by MALDI-TOF and the disk-diffusion method, respectively. Of 365 isolates, Enterococcus hirae was the most prevalent, being more likely to be isolated from faeces than from milk (odds ratio (OR) = 39 center dot 2), liners (OR = 5 center dot 4) or bedding (OR = 2 center dot 2). Enterococcus saccharolyticus was the most prevalent in milk samples. The chances of isolating Enterococcus faecalis from milk were higher than from aisles (OR = 12 center dot 5), faeces (OR = 5 center dot 3), bedding (OR = 3 center dot 6) or liners (OR = 3 center dot 0). The odds of isolating Enterococcus faecium from faeces were higher than from liners (OR = 7 center dot 3), bedding (OR = 2 center dot 5) or aisles (OR = 2 center dot 4). Of 360 tested isolates, 1 center dot 9, 0 center dot 3 and 0 center dot 6% were resistant to penicillin, vancomycin and teicoplanin, respectively. Our results suggest that Enterococcus species can occupy specific ecological niches on dairy farms and pose a risk to public and animal health. (AU)

• Processo FAPESP: 15/19688-8 - E. coli, Klebsiella pneumoniae e Enterococcus spp: impacto dos fatores de virulência na mastite bovina e reflexos na saúde pública
• Beneficiário: Helio Langoni
• Modalidade de apoio: Auxílio à Pesquisa - Temático