Amino acid decorated xanthan gum coatings: Molecular arrangement and cell adhesion

Autor(es)

Alavarse, Alex Carvalho ; Frachini, Emilli Caroline G. ; Silva, Jean Bezerra ; Pereira, Rafael dos Santos ; Ulrich, Henning ; Siqueira Petri, Denise Freitas

• Tipo de documento: Artigo Científico
• Fonte: CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS; v. 4, p. 11-pg., 2022-06-13.

Resumo

In this work, citric acid molecules mediated simultaneously the crosslinking of xanthan gum (XG) chains and the grafting of glutamic acid (Glu), cysteine (Cys), histidine (His) or tryptophan (Trp) to XG chains, as evidenced by Fourier-transform infrared spectroscopy, elemental and thermogravimetric analyses, and high-resolution X-ray photoelectron spectroscopy. XG coatings (similar to 60 nm thick) presented total surface energy (gamma S) of 65 mJ/m(2). The attachment of amino acids to the XG chains decreased.S values to similar to 48 mJ/m(2) because the surface energy polar (gamma S-p) component decreased. Circular dichroism (CD) spectra revealed that the helix conformation of XG chains was retained in XG and XG-Glu-coatings, but it was lost in the XG-His, XG-Cys-and XG-Trp-coatings due to intermolecular interactions between the amino acid side groups (thiol, imidazole, and indole). XG-based coatings presented no cytotoxicity. After 3 h of incubation, the adhesion of SH-5YSY neural cells in comparison to the control (100%) was XG-His (18%), XG-Cys (21.4%), XG (29%), XG-Trp (28.6%) and XG-Glu (46.4%). The decrease of% cell adhesion tended to be favored by the decrease of gamma S-p, except for XG-Glu-and XG-Trp, and by the loss of helix molecular conformation of chains in the coatings. (AU)

• Processo FAPESP: 20/01907-3 - Determinação das propriedades mecânicas de arcabouços magnéticos a base de polissacarídeos
• Beneficiário: Alex Carvalho Alavarse
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/07366-4 - Receptores de purinas e cininas como alvos de estudo e intervenção terapêutica em doenças neurológicas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/13492-2 - Arcabouços sintéticos e naturais aplicados à medicina regenerativa
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático