| Texto completo | |
| Autor(es): |
Remonatto, Daniela
;
Fantatto, Rafaela Regina
;
Pietro, Rosemeire Cristina Linhari Rodrigues
;
Monti, Rubens
;
Oliveira, J. Vladimir
;
de Paula, Ariela Veloso
;
Bassan, Juliana Cristina
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Process Biochemistry; v. 120, p. 14-pg., 2022-06-27. |
| Resumo | |
Geraniol esters, such as geranyl acetate, are known to have antimicrobial and insect-repellent action, but their acaricidal activity has been little explored. This study aimed to investigate the solvent-free synthesis of geranyl acetate in batch (BSTR) and fed-batch (FBSTR) stirred tank reactors using the immobilized lipases NS 88011 and Novozym 435 and evaluate its larvicidal activity against the cattle tick Rhipicephalus (Boophilus) microplus. A central composite design (CCD) was used to maximize the synthesis of geranyl acetate in a BSTR. The independent variables were temperature reaction (40-60 degrees C), acetic acid/geraniol molar ratio (1:1-1:3), and enzyme loading (1-5% w/w in relation to the substrates). Novozym 435 yielded the best results, achieving 99% of geranyl acetate conversion using 1:3 acetic acid/geraniol molar ratio, 60 degrees C and 5% (w/w) of enzyme loading after 1 h of reaction in a BSTR. In the FBSTR system, a high content of geranyl acetate was obtained (about 80%) in 4 h of reaction using a 1:1 acetic acid/geraniol molar ratio, 60 degrees C and 5% (w/w) Novozym 435. Geranyl acetate showed potential as an acaricide, with an LC90 of 6.25 mg/mL and 100% mortality at a concentration of 12.5 mg/mL. (AU) | |
| Processo FAPESP: | 20/09592-1 - Ésteres bioativos nanoencapsulados: síntese enzimática e estudo do potencial antimicrobiano |
| Beneficiário: | Ariela Veloso de Paula |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |