| Texto completo | |
| Autor(es): |
Ma, Hui L.
;
Li, Wanlu
;
Wang, Mian
;
Varanda, Laudemir C.
;
Perussi, Janice R.
;
Zhang, Y. Shrike
;
Carrilho, Emanuel
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIO-DESIGN AND MANUFACTURING; v. 5, n. 4, p. 14-pg., 2022-07-23. |
| Resumo | |
Advances in biomimetic three-dimensional (3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating in vivo tumor architectures is more accurate than traditional two-dimensional (2D) cell culture. Gelatin methacryloyl (GelMA) is widely used as a tissue-engineered scaffold hydrogel for 3D cell culture. In the present study, an in vitro 3D malignant melanoma model based on GelMA was fabricated to evaluate the efficiency of hypericin (Hy)-loaded microemulsion (ME) in photodynamic therapy against melanoma. The ME was produced by the spontaneous emulsification method to enhance the bioavailability of Hy at tumor sites. Hy-loaded MEs were applied to a 3D malignant melanoma model made using 6% GelMA and the co-culture of B16F10 and Balb/c 3T3 cells, followed by crosslinking using violet light (403 nm). The observation revealed excellent cell viability and the presence of F-actin cytoskeleton network. Hy-loaded MEs exhibited higher phototoxicity and cell accumulation (about threefold) than free Hy, and the cells cultured in the 3D system displayed lower susceptibility (about 2.5-fold) than those in 2D culture. These findings indicate that the developed MEs are potential delivery carriers for Hy; furthermore, GelMA hydrogel-based modeling in polydimethylsiloxane (PDMS) molds is a user-friendly and cost-effective in vitro platform to investigate drug penetration and provide a basis for evaluating nanocarrier efficiency for skin cancer and other skin-related diseases. (AU) | |
| Processo FAPESP: | 13/01284-2 - Nanopartículas teranósticas a base de óxido de ferro superparamagnético para terapia de câncer da pele via hipertermia magnética |
| Beneficiário: | Laudemir Carlos Varanda |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |