Synergism for xylo-oligosaccharides, p-coumaric and ferulic acid production, and thermostability modulation of GH 62 alpha-L-arabinofuranosidase

Autor(es)

Martins, Manoela ; Tramontina, Robson ; Squina, Fabio Marcio ; Dinamarco, Taisa Magnani ; Goldbeck, Rosana

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 44, p. 11-pg., 2022-08-26.

Resumo

GH 62 alpha-L-arabinofuranosidases are a group of enzymes that preferably hydrolyze L-arabinose residues of arabinoxylans. Albeit the least studied, these enzymes have gained attention because they exert synergistic action with other xylanolytic enzymes for arabinoxylan depolymerization. This is the first study on the thermodynamic and kinetic parameters, structural prediction, and metal ion effect on the thermostability of a GH 62 arabinofuranosidase subfamily 1. The optimum enzymatic conditions are pH 4.8 and 42 C, and the presence of Ca2+ and Mn2+ ions enhanced the thermostability and activity. The synergism of this arabinofuranosidase with a GH 10 endox-ylanase increased the production of xylo-oligosaccharides 2.3 times with additional coproduction of 0.82 mM of ferulic acid and 0.09 mM of p-coumaric acid released from corn hull arabinoxylan (4% w/v). These results are relevant to developing an enzymatic formulation that is economically feasible for industrial applications, especially for prebiotic and antioxidant compounds produc-tion in the food and pharmaceutical sectors from sources rich in arabinoxylan, especially agro-industrial wastes of grains husks and peels. (AU)

• Processo FAPESP: 20/02871-2 - Proteína quimérica bifuncional de endoxilanase/arabinofuranosidase para hidrólise de resíduos agroindustriais e produção de xilo-oligossacarídeos
• Beneficiário: Manoela Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/50590-4 - Valorização da lignina em plantas de etanol celulósico: a conversão biocatalítica via ácido ferúlico a produtos químicos de alto valor
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Regular