| Texto completo | |
| Autor(es): |
Zanona, Querusche Klippel
;
Marconi, Gabriel Alves
;
Couto Pereira, Natividade de Sa
;
Lazzarotto, Gabriela
;
Ferreira Donatti, Ana Luiza
;
Cortes de Oliveira, Jose Antonio
;
Garcia-Cairasco, Norberto
;
Calcagnotto, Maria Elisa
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Neuroscience; v. 500, p. 15-pg., 2022-08-17. |
| Resumo | |
Wistar Audiogenic Rats (WAR) is an inbred rodent strain susceptible to acute auditory stimulation-induced seizures. However, spontaneous epileptic seizures (SES) and their associated electroencephalogram (EEG) abnormalities have not been reported in WAR kindled animals. The same is true for naive WARs (without sound-induced seizures). An approach to increment epileptogenesis and SES is to use a second insult to be added to the genetic background. Here, we used adult naive WARs with microgyria induced by neonatal cortical freeze-lesion (FL) to evaluate the occurrence of SES and the modification in cortical oscillation patterns and behavior. The neonatal cortical FL was performed in Wistar and naive WARs (Wis-FL and WAR-FL). Sham animals were used as controls (Wistar-S and WAR-S). Video-EEG recordings and behavioral tasks were performed during adulthood. Surprisingly, spike-waive discharges (SWD) events associated with behavior arrest were detected in WAR-S rats. Those events increased in duration and number in WAR-FL animals. The EEG quantitative analysis showed decreased power of cortical delta, theta and beta oscillations in WAR-S, decreased power of cortical fast gamma (FG) oscillations in WARs, independent of microgyria, and decreased interhemispheric synchrony for delta and FG with stronger coupling in delta and theta-FG oscillations in FL animals. The WARs, regardless of microgyria, had reduced locomotor activity, but only WAR-FL animals had reduced anxiety-like behavior. Microgyria in naive WARs intensified SWD events associated with behavior arrest that could reflect absence-like seizures and abnormal cortical oscillations, and reduced anxiety-like behavior indicating that WAR-FL could be a reliable model to study epileptogenesis. (C) 2022 IBRO. Published by Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 19/05957-8 - Epilepsias e comorbidades neuropsiquiátricas: caracterização dos efeitos do tratamento com canabidiol e HUF-101 em modelos experimentais in vivo e in vitro |
| Beneficiário: | Norberto Garcia Cairasco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |