| Texto completo | |
| Autor(es): Mostrar menos - |
de Oliveira Neto, Nilson Ferreira
;
Sena Bonvicini, Jessica Fernanda
;
de Souza, Gabriela Leite
;
Santiago, Mariana Brentini
;
Sola Veneziani, Rodrigo Cassio
;
Ambrosio, Sergio Ricardo
;
Bastos, Jairo Kenupp
;
Barbosa Silva, Marcelo Jose
;
Gomes Martins, Carlos Henrique
;
Gomes Moura, Camilla Christian
;
Turrioni, Ana Paula
Número total de Autores: 11
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| Tipo de documento: | Artigo Científico |
| Fonte: | ARCHIVES OF ORAL BIOLOGY; v. 143, p. 11-pg., 2022-08-29. |
| Resumo | |
Objective: This study aimed to evaluate the antibacterial activity of crude Brazilian red propolis (BRP) extract against anaerobic bacteria involved in primary endodontic infection. Additionally, we evaluate the cell viability and free radical production of human dental pulp fibroblasts (HDPF) in direct contact with mineral trioxide aggregate (MTA) and BRP. Design: The Minimum Inhibitory Concentration, Minimum Bactericidal Concentration (MIC, MBC) and Minimum Inhibitory Concentration of Biofilm (MICB50) of BRP against anaerobic endodontic pathogens were determined. HDPF were exposed to BRP10 (10 mu g/mL), BRP50 (50 mu g/mL), MTA extract (1:1, 1:2, 1:4 e 1:8), dimethyl sulfoxide 0.5% (DMSO), and cell culture medium (DMEM). The groups were tested for cell viability (MTT assay), and free radical production (reactive oxygen species -ROS, DCFH-DA probe and nitric oxide - NO, Griess re-agent). The one-way ANOVA and Tukey's tests were employed at a significance level of 5%. Results: MIC/MBC values of BRP performed antibacterial activity for Parvimonas micra (6.25/6.25 mu g/mL), Fusobacterium nucleatum (25/25 mu g/mL), Prevotella melaninogenica (50/100 mu g/mL), Prevotella nigrescens (50/100 mu g/mL), Prevotella intermedia (50/100 mu g/mL), and Porphyromonas gingivalis (50/200 mu g/mL). The MICB50 values ranged from 1.56 to 50 mu g/mL. BRP and MTA stimulated cell viability, emphasizing BRP10 (p = 0.007). Furthermore, it was observed that MTA 1:1, MTA 1:2, and BRP50 slightly increased ROS (p < 0.001) and NO production (p = 0.008, p = 0.007, and p < 0.001 respectively) compared to DMEM group. Conclusions: BRP exhibits good antibacterial activity against endodontic pathogens, and both BRP and MTA promote the viability of HDPF without increasing NO and ROS production. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |