Simulated gastrointestinal digestion/Caco-2 cell model to predict bioaccessibility and intestinal permeability of p-coumaric acid and p-coumaroyl derivatives in peanut

Autor(es)

Massarioli, Adna Prado ; Sartori, Alan Giovanini de Oliveira ; Juliano, Fernanda Francetto ; do Amaral, Jose Eduardo Pedroso Gomes ; dos Santos, Roseane Cavalcanti ; de Lima, Liziane Maria ; de Alencar, Severino Matias

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 400, p. 8-pg., 2023-01-30.

Resumo

Data concerning physiological recovery of whole peanut major phenolics throughout the gastrointestinal tract are scarce. In our study, the bioaccessibility and intestinal permeability of peanuts major phenolics were pre-dicted by simulated digestion followed by Caco-2 cells monolayer model. Phenolics identification and quanti-fication were performed by HPLC-ESI-QTOF-MS and HPLC-PDA, respectively. As results, p-coumaroyl conjugates with tartaric, sinapic and ferulic acids, and p-coumaric acid were the major phenolics found in the non-digested extract and in the digested and transported fractions. The in vitro bioaccessibility and Caco-2 cell transport of p-coumaric acid was 370% and 127%, respectively, while it was much lower for p-coumaroyl derivatives (7-100% and 14-31%, respectively). Nonetheless, the peroxyl scavenging activity remained unaltered, likely, at least partly, due to synergies between some phenolics, which concentration proportions changed throughout the experiment. Hence, there is indication that whole peanut is a source of bioavailable antioxidant phenolics. (AU)

• Processo FAPESP: 16/06514-4 - Composição química e potencial de desativação de espécies reativas de oxigênio de novas cultivares de amendoim adaptadas a ambientes semiáridos: uma contribuição em conjunto com a Embrapa ao desenvolvimento econômico/social do Nordeste brasileiro
• Beneficiário: Severino Matias de Alencar
• Modalidade de apoio: Auxílio à Pesquisa - Regular