| Texto completo | |
| Autor(es): Mostrar menos - |
Moraes, Gustavo Simao
;
Albach, Thais
;
Sugio, Carolina Yoshi Campos
;
Cachoeira, Victoria Schlumberger
;
Kiratcz, Falyne
;
Claudino, Marcela
;
Campagnoli, Eduardo Bauml
;
Pochapski, Marcia Thais
;
dos Santos, Fabio Andre
;
Neppelenbroek, Karin Hermana
;
Urban, Vanessa Migliorini
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Medical Mycology; v. 60, n. 12, p. 11-pg., 2022-11-06. |
| Resumo | |
Lay Summary Denture stomatitis is an oral inflammatory disease with high recurrence rates. Different animal models have been reported in the literature, but some gaps still need to be addressed. A reproducible in vivo model should be established to test new treatment approaches. This study compared different conditions to establish a rat model of denture stomatitis. Immunocompetent Wistar rats were divided into two groups (n = 35): Tetracycline = administration of 0.83 mg/ml of tetracycline hydrochloride 7 days before induction of denture stomatitis and amoxicillin = administration of 0.156 mg/ml of amoxicillin with clavulanic acid 4 days before induction of denture stomatitis. A suspension of Candida albicans was inoculated on the palate followed by the use of a palatal device contaminated with C. albicans inoculum for 4 days to induce denture stomatitis. As controls, some rats were not submitted to any procedure or used a sterile palatal device for 4 days. The development of denture stomatitis was confirmed by visual analysis, colony-forming units per milliliter (CFU/ml) count, histopathological and immunohistochemical analyses, and through myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats were euthanized right after device removal (T0), 4 (T4), or 6 (T6) days after device removal. Tetracycline improved the development of the disease, with more severe clinical signs at T0. Similar results were observed in the CFU/ml count and in the histometric and immunohistochemical analyses. Higher MPO expression was detected in the palates of the tetracycline group (P = .006). Despite the subtle differences between antibiotics, tetracycline showed better results in inducing and maintaining denture stomatitis for at least 4 days after device removal. (AU) | |
| Processo FAPESP: | 17/07314-1 - Avaliação clínica da adição de antifúngicos a reembasador resiliente temporário no tratamento da estomatite protética |
| Beneficiário: | Karin Hermana Neppelenbroek |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/22996-1 - Resposta tecidual em modelo animal de condicionador de tecido contendo complexo de inclusão antifúngico: ²-ciclodextrina para tratamento de estomatite protética |
| Beneficiário: | Carolina Yoshi Campos Sugio |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |