Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study

Autor(es)

de Macedo, Erenildo Ferreira ; Santos, Nivia Salles ; Nascimento, Lucca Silva ; Mathey, Raphael ; Brenet, Sophie ; de Moura, Matheus Sacilotto ; Hou, Yanxia ; Tada, Dayane Batista

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 1, p. 20-pg., 2023-01-01.

Resumo

Regardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle and the biochemical environment, numerous studies have provided evidence that direct contact between NPs and biomolecules or cell membranes leads to cell inactivation or damage and may be a primary mechanism for cytotoxicity. In such a context, this work focused on developing a fast and accurate method to characterize the interaction between NPs, proteins and lipidic membranes by surface plasmon resonance imaging (SPRi) technique. The interaction of gold NPs with mimetic membranes was evaluated by monitoring the variation of reflectivity after several consecutive gold NPs injections on the lipidic membranes prepared on the SPRi biochip. The interaction on the membranes with varied lipidic composition was compared regarding the total surface concentration density of gold NPs adsorbed on them. Then, the interaction of gold and silver NPs with blood proteins was analyzed regarding their kinetic profile of the association/dissociation and dissociation constants (k(off)). The surface concentration density on the membrane composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and cholesterol (POPC/cholesterol) was 2.5 times higher than the value found after the injections of gold NPs on POPC only or with dimethyldioctadecylammonium (POPC/DDAB). Regarding the proteins, gold NPs showed preferential binding to fibrinogen resulting in a value of the variation of reflectivity that was 8 times higher than the value found for the other proteins. Differently, silver NPs showed similar interaction on all the tested proteins but with a variation of reflectivity on immunoglobulin G (IgG) 2 times higher than the value found for the other tested proteins. (AU)

• Processo FAPESP: 17/01697-6 - Síntese e funcionalização de nanopartículas bimetálicas para aplicação na terapia fotodinâmica de câncer
• Beneficiário: Dayane Batista Tada
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/06803-4 - Desenvolvimento de biossensor para câncer de ovário baseado na técnica de SPRi
• Beneficiário: Erenildo Ferreira de Macedo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/14795-3 - Avaliação da afinidade entre nanopartículas metálicas e proteínas do soro por ressonância plasmônica de superfície com imagens
• Beneficiário: Nivia Salles Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/07354-0 - Desenvolvimento de biossensor para biomarcadores de Câncer de ovário baseado na técnica de SPRi
• Beneficiário: Erenildo Ferreira de Macedo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/00807-5 - Aplicação nanopartículas bimetálicas na detecção simultânea de proteínas em solução multicomponentes por ressonância plasmônica de superfície
• Beneficiário: Lucca Silva Nascimento
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/20503-5 - Avaliação da afinidade entre nanopartículas metálicas e proteínas do soro por ressonância plasmônica de superfície com imagens
• Beneficiário: Nivia Salles Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica