Adjuvant effect of mesoporous silica SBA-15 on anti-diphtheria and anti-tetanus humoral immune response

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Trezena, Aryene Goes ; Oseliero Filho, Pedro Leonidas ; da Silva, Luis Carlos Cides ; Oliveira, Cristiano Luis Pinto ; Lopes, Jose Luiz de Souza ; Antonio, Nayara da Silva ; Dettmann, Viktor Fonseca Barbosa ; Akamatsu, Milena Apetito ; Martins, Tereza da Silva ; Ribeiro, Orlando Garcia ; Fantini, Marcia Carvalho de Abreu ; Sant'Anna, Osvaldo Augusto ; Tino-De-Franco, Milene

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICALS; v. 80, p. 9-pg., 2022-10-01.

Resumo

Routine immunization against diphtheria and tetanus has drastically reduced the incidence of these diseases worldwide. Anti-diphtheria/tetanus vaccine has in general aluminum salt as adjuvant in its formulation that can produce several adverse effects. There is a growing interest in developing new adjuvants. In this study, we evaluated the efficiency of SBA-15 as an adjuvant in subcutaneous immunization in mice with diphtheria (dANA) and tetanus (tANA) anatoxins as well as with the mixture of them (dtANA). The tANA molecules and their encapsulation in SBA-15 were characterized using Small-Angle X-ray Scattering (SAXS), Dynamical Light Scat-tering (DLS), Nitrogen Adsorption Isotherm (NAI), Conventional Circular Dichroism (CD)/Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy, and Tryptophan Fluorescence Spectroscopy (FS). The primary and secondary antibody response elicited by subcutaneous immunization of High (HIII) and Low (LIII) antibody responder mice with dANA, tANA, or dtANA encapsulated in the SBA-15 were determined. We demonstrated that SBA-15 increases the immunogenicity of dANA and tANA antigens, especially when administered in combina-tion. We also verified that SBA-15 modulates the antibody response of LIII mice, turning them into high antibody responder. Thus, these results suggest that SBA-15 may be an effective adjuvant for different vaccine formulations. (AU)

• Processo FAPESP: 17/17844-8 - Sílica nanoestruturada como veículo protetor de vacinas e biomoléculas
• Beneficiário: Osvaldo Augusto Brazil Esteves Sant'Anna
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/13204-7 - O papel da morfologia da sílica mesoporosa na incorporação das anatoxinas de difteria e tétano: potenciais adjuvantes para vacinas orais
• Beneficiário: Pedro Leonidas Oseliero Filho
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 19/12301-1 - Síntese de sílicas mesoporosas ordenadas com diferentes estruturas e morfologias
• Beneficiário: Pedro Leonidas Oseliero Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado