Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

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Fernandes, Mileni S. ; Sabino-Arias, Isabela T. ; Dionizio, Aline ; Fabricio, Mayara F. ; Trevizol, Juliana S. ; Martini, Tatiana ; Azevedo, Liane B. ; Valentine, Ruth A. ; Maguire, Anne ; Zohoori, Fatemeh, V ; Amaral, Sandra L. ; Buzalaf, Marilia A. R.

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 12, n. 2, p. 22-pg., 2022-02-01.

Resumo

We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI; RI) No-F, No-Exercise, (SII; RII) 50 ppm F, No-Exercise, (SIM RIII) 50 ppm F, Exercise. Overall, mean F concentrations in the plasma and femur were significantly higher in R-mice compared with S-mice. In R-mice, exercise resulted in an increase in F accumulation in the femur. In S-mice, the mean plasma glucose level was significantly higher in Group II compared with Groups I and III. There was an increase in liver proteins involved in energy flux and antioxidant enzymes in non-exercise groups (I, II) of S-mice in comparison with the corresponding groups of R-mice. The results also showed a decrease in muscle protein expression in Group I S-mice compared with their R-mice counterparts. In conclusion, the findings suggest an increased state of oxidative stress in fluorosis-susceptible mice that might be exacerbated by the treatment with F. In addition, fluorosissusceptible mice have plasma glucose levels higher than fluorosis-resistant mice on exposure to F, and this is not affected by exercise. (AU)

• Processo FAPESP: 16/20020-4 - Influências genéticas e ambientais no metabolismo e retenção do fluoreto
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Sandra Lia do Amaral Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 14/50798-1 - Fluoride (F) metabolism/body F retention: effects of host (genetic variation) and environment (F dose and exercise) factors
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/12109-2 - Efeito do exercício físico no metabolismo de fluoretos em camundongos com diferentes susceptibilidades à fluorose: análise proteômica.
• Beneficiário: Mileni da Silva Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/05278-2 - Papel do sistema renina-angiotensina e do sistema nervoso simpático na hipertensão induzida por dexametasona: efeitos preventivos do exercício físico
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• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico