| Texto completo | |
| Autor(es): |
Castaneda, Carol
;
Bravo, Karent
;
Cortes, Natalie
;
Bedoya, Janeth
;
Borges, Warley de S.
;
Bastida, Jaume
;
Osorio, Edison
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF APPLIED BIOMEDICINE; v. 21, n. 1, p. 12-pg., 2023-03-31. |
| Resumo | |
Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 mu g/ml) had a clear effect on the viability of melanoma cells, reducing it by 45.7% without affecting healthy keratinocytes. This alkaloid fraction and tazettine (both at 2.5 mu g/ml) suppressed UVB-induced ROS production by 31.6% and 29.4%, respectively. The highest anti-inflammatory potential was shown by the Zephyranthes carinata (bulb) alkaloid fraction (10 mu g/ml), which reduced IL-6 production by 90.8%. According to the chemometric analysis, lycoramine and tazettine had a photoprotective effect on the UVB-exposed HaCaT cells, attenuating the production of ROS and IL-6. These results suggest that Amaryllidaceae alkaloids have photoprotective and therapeutic potential in skin cancer management, especially at low concentrations. (AU) | |
| Processo FAPESP: | 14/50926-0 - INCT 2014: biodiversidade e produtos naturais |
| Beneficiário: | Vanderlan da Silva Bolzani |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |