Aptasensing of beta-amyloid (Aβ_(1-42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites

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Negahdary, Masoud ; Veloso, William Barros ; Bacil, Raphael Prata ; Buoro, Rafael Martos ; Gutz, Ivano Gebhardt Rolf ; Paixao, Thiago Regis Longo Cesar ; do Lago, Claudimir Lucio ; Sakata, Solange Kazumi ; Meloni, Gabriel Negrao ; Franca, Mesaque Carvalho ; de Oliveira, Thawan Gomes ; Ameku, Wilson Akira ; Durazzo, Michelangelo ; Angnes, Lucio

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 393, p. 11-pg., 2023-10-15.

Resumo

In this study, beta-amyloid (A beta((1-42))), an essential biomarker for diagnosing Alzheimer's disease (AD), was detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: similar to 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times, compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created an optimized biointerface to detect A beta((1-42)) in a linear range from 0.1 pg mL(-1) to 10 ng mL(-1) (limit of detection (LOD): 84 fg mL(-1), (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and human serum. (AU)

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