Optimization of biomass saccharification processes with experimental design tools for 2G ethanol production: a review

Autor(es)

Bernardi, Aline V. ; Gerolamo, Luis E. ; Dinamarco, Taisa Magnani ; Tapia-Blacido, Delia Rita

• Tipo de documento: Artigo Científico
• Fonte: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. N/A, p. 18-pg., 2023-08-31.

Resumo

The current energy scenario has encouraged the replacement of carbon sources with renewable sources. Second-generation (2G) ethanol production from lignocellulosic material or agroindustrial biomass involves a three-step process consisting of pretreatment, saccharification and fermentation. Enzymatic saccharification is the most critical step for recovering fermentable sugars from biomass. Many factors such as pretreatment, enzyme load, pH, temperature, substrate and surfactant concentrations, and reaction time, among others, can affect biomass saccharification. In this sense, different design of experiment (DoE) tools together with response surface methodology (RSM) have been used as a viable strategy to determine how factors influence saccharification and to optimize this process. The strategy involves fewer experiments, less time and lower cost. This review summarizes how DoE and RSM tools have been used to optimize biomass saccharification. Moreover, an overview of the main experimental design tools used in this field is provided. (AU)

• Processo FAPESP: 19/06329-0 - Aproveitamento de resíduos agroindustriais para a produção de bandejas biodegradáveis e bioativas
• Beneficiário: Delia Rita Tapia Blácido
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/19095-0 - Identificação, caracterização e expressão funcional de enzimas auxiliares hidrolíticas do tipo LPMOs do fungo Aspergillus fumigatus
• Beneficiário: Taisa Magnani Dinamarco
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular