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Nutrient Optimization Strategy to Increase the Carbohydrate Content of Chlorella vulgaris and Evaluation of Hydrolysis and Fermentation Performance

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Autor(es):
Silvello, Maria Augusta de Carvalho ; Gasparotto, Giovanna Alves ; Ferreira, Gabriela F. ; Santos, Lucielen Oliveira ; Fregolente, Leonardo V. ; Goldbeck, Rosana
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: BioEnergy Research; v. N/A, p. 10-pg., 2023-09-06.
Resumo

Carbohydrate content of microalgae biomass can be increased and modulated. The nutrient limitation strategy may be promising when employed in a two-stage process, to limit carbohydrate productivity compromising biomass production. In this study, the effects of pH, nitrogen, sulfur, and phosphorus concentration on Chlorella vulgaris growth and carbohydrate accumulation were evaluated. Carbohydrate content reached 58% on the 14th cultivation day, with a biomass concentration of 0.876 +/- 0.037 g/L, when microalga was cultured in modified BG-11 medium (35 mg/L K2HPO4, 80 MgSO4 center dot 7H(2)O mg/L, nitrogen-depleted and pH 9). Under these conditions, carbohydrate productivity was enhanced by 72.04%, in comparison to the standard medium. In addition, the sugar profile of the microalgae cultivated in the modified nitrogen-depleted medium was altered, displaying a shift in microalgae metabolism towards carbohydrate accumulation. The hydrolysis and fermentation performance of the carbohydrate-rich microalgae biomass was assessed through different methods for process endorsement, and resulted in high reducing sugars release (5.48 g/L of glucose from 10 g/L of biomass), and theoretical ethanol yield (69%), highlighting the impact and feasibility of bioethanol 3G production. (AU)

Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
Beneficiário:Rosana Goldbeck Coelho
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/20787-9 - Estratégias para sacarificação de biomassa microalgal visando a produção de bioetanol 3G
Beneficiário:Maria Augusta de Carvalho Silvello
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade
Beneficiário:Rubens Maciel Filho
Modalidade de apoio: Auxílio à Pesquisa - Temático