Self-assembled peptide P11-4 interacts with the type I collagen C-terminal telopeptide domain and calcium ions

Autor(es)

Carvalho, Rafael Guzella ; Patekoski, Luiz Fernando ; Puppin-Rontani, Regina M. ; Nakaie, Clovis Ryuichi ; Nascimento, Fabio Dupart ; Tersariol, Ivarne L. S.

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 39, n. 8, p. 10-pg., 2023-08-01.

Resumo

Objectives: Evaluate molecularly the role of P-11-4 self-assembly peptide in dentin remineralization and its interaction with collagen I. Methods: The calcium-responsive P-11-4 peptide was analyzed by intrinsic fluorescence emission spectrum, circular dichroism spectrum (CD), and atomic force microscope (AFM). Differential light scattering was used to monitor the nucleation growth rate of calcium phosphate nanocrystals in the absence or in the presence of P-11-4. AFM was used to analyze the radial size (nm) of calcium phosphate nanocrystals formed in the absence or in the presence of P-11-4, as well as to verify the spatial structure of P-11-4 in the absence or in the presence of Ca2+. Results: The interaction of Ca2+ with the P-11-4 (K-D = 0.58 +/- 0.06 mM) promotes the formation of beta-sheet antiparallel structure, leads to its precipitation in saturated solutions of Ca/P = 1.67 and induces the formation of parallel large fibrils (0.6 - 1.5 mu m). P-11-4 organized the HAP nucleation by reducing both the growth rate and size variability of nanocrystals, analyzed by the F test (p < 0.0001, N = 30). P-11-4 interacts (K-D = 0.75 +/- 0.06 mu M) with the KGHRGFSGL motif present at the C-terminal collagen telopeptide domain. P-11-4 also increased the amount of HAP and collagen in the MDPC-23 cells. Significance: The presented data propose a mechanism that will help future clinical and/or basic research to better understand a molecule able to inhibit structural collagen loss and help the impaired tissue to remineralize. (AU)

• Processo FAPESP: 15/03964-6 - Glicosaminoglicanos e proteoglicanos: relação estrutura e função
• Beneficiário: Helena Bonciani Nader
• Modalidade de apoio: Auxílio à Pesquisa - Temático