Synergic vascular photodynamic activity by methylene blue-curcumin supramolecular assembly

Autor(es)

Silva, Rodrigo C. ; Buzza, Hilde H. ; Ducas, Eli S. A. ; Oliveira, Kleber T. ; Bagnato, Vanderlei S. ; Souza, Guilherme R. L. ; Almeida, Luciane M. ; Goncalves, Pablo J.

• Tipo de documento: Artigo Científico
• Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 303, p. 10-pg., 2023-12-15.

Resumo

A supramolecular assembly was obtained by combining methylene blue (MB) with a natural plant extract, curcumin (Curc), in a stoichiometric ratio of 1:4 in aqueous solution (90% PBS + 10% ethanol) at room temperature. The MB-Curc supramolecular assembly was evidenced by absorption and fluorescence spectroscopies, and the stoichiometry and bonding constant were obtained using Cielens model. Its stability and photostability were evaluated by chromatographic analysis and UV-Vis absorption. The MB-Curc avoids the aggregation of both isolated compounds and efficiently produces singlet oxygen (& phi;& UDelta;= 0.52 & PLUSMN; 0.03). Its potential for photodynamic antiangiogenic treatments was evaluated through the vascular effect observed in chicken chorioallantoic membrane (CAM) assay. The results showed intense damage in CAM vascular network by MB-Curc after irradiation, which is higher than the effect of isolated compounds, indicating a synergistic vascular effect. This combination can be essential to prevent cancer revascularization after photodynamic application and improve the efficacy of this approach. The characteristics exhibited by MB-Curc make it a potential candidate for use in cancer treatments through photodynamic antiangiogenic therapy. (AU)

• Processo FAPESP: 18/00879-6 - Reações de Arilação de Heterociclos Fotocatalisadas por Porfirinas em Condições de Batelada e em Regime de Fluxo Contínuo
• Beneficiário: Rodrigo Costa e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado