Electrochemical detection of xylobiose in banana biomass using a 3D porous copper oxide foam electrode modified with a molecularly imprinted Poly-L-arginine film

Autor(es)

da Silva, Mateus Paula ; Beluomini, Maisa Azevedo ; de Freitas, Carolina ; Brienzo, Michel ; Stradiotto, Nelson Ramos

• Tipo de documento: Artigo Científico
• Fonte: Journal of Food Composition and Analysis; v. 124, p. 10-pg., 2023-12-01.

Resumo

Xylobiose (X2) presents numerous health benefits, including cancer prevention, and can be found in agroindustrial biomass, including bananas. Here, an electrochemical sensor based on molecularly imprinted polyL-arginine film (MIP) and 3D porous copper oxide foam (3DnpCu) for the ultrasensitive detection of X2 is studied. Te MIP/3DnpCu-GCE is prepared by dynamic hydrogen bubble template method, followed by electropolymerization of L-arginine in the presence of X2 as template, followed by electrochemical extraction, resulting in the formation of X2 recognition sites. The sensor was characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. MIP/3DnpCu-GCE showed a wide linear response in the concentration range of 1.0 x 10-12 to 1.0 x 10-10 mol L-1, limit of detection of 7.7 x 10-13 mol L-1 and sensibility of 1.4 mu A pmol-1 L. The sensor exhibited selective X2 recognition, long-term stability (maintaining 86% of its initial current over 8 days), while inter-electrode repeatability displayed an RSD of 2.4%. The applicability of the MIP/3DnpCu-GCE in real samples is demonstrated by successfully quantifying X2 concentration in banana biomass. Furhermore, the comparison between the data obtained using this method and those found by the HPLC method confirmed the accuracy of the sensor. (AU)

• Processo FAPESP: 18/12131-6 - Sensores eletroquímicos baseados em eletrodos impressos modificados com nanoporos metálicos e monitorados por rede sem fio para determinação de flavonóides nos resíduos da indústria cítrica
• Beneficiário: Maísa Azevedo Beluomini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/02550-4 - Sensor eletroquímico baseado em nanoporos de cobre e polímero molecularmente impresso para a determinação de xilobiose em casca e polpa de banana.
• Beneficiário: Mateus Paula da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/22401-8 - Fruto-refinaria: processos de obtenção, métodos de caracterização e geração de produtos oriundos de resíduos da fruticultura
• Beneficiário: Nelson Ramos Stradiotto
• Modalidade de apoio: Auxílio à Pesquisa - Temático