Bioreactor Production Process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide

Autor(es)

Klafke, Karina ; Sanches, Marcio Martinello ; Sihler, William ; de Souza, Marlinda Lobo ; Tonso, Aldo

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 12, n. 8, p. 13-pg., 2023-08-01.

Resumo

Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10(11) OBs/L. (AU)

• Processo FAPESP: 17/06371-1 - Desenvolvimento de processo de produção de bioinseticida baculovírus por células de inseto Sf9 em biorreator
• Beneficiário: Karina Klafke
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/08526-5 - Produção de baculovírus comparando biorreatores STR e wave
• Beneficiário: Karina Klafke
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado