| Texto completo | |
| Autor(es): |
Barbieri, Giorgia S.
;
Bento, Heitor B. S.
;
de Oliveira, Fernanda
;
Picheli, Flavio P.
;
Dias, Lidia M.
;
Masarin, Fernando
;
Santos-Ebinuma, Valeria C.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOTECH; v. 11, n. 2, p. 12-pg., 2022-05-17. |
| Resumo | |
In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with industrial applicability. In this work, xylanase production by the filamentous fungi Talaromyces amestolkiae in submerged culture was evaluated using five agroindustrial byproducts, namely, wheat bran, citrus pulp, rice bran, peanut skin, and peanut shell. Firstly, the aforementioned byproducts were characterized in terms of cellulose, xylan, lignin, and extractives. Next, production studies were performed, and wheat bran generated the highest enzymatic activity (5.4 UmL(-1)), probably because of its large amount of xylan. Subsequently, a factorial design was performed to evaluate the independent variables yeast extract, wheat bran, K2HPO4, and pH, aiming to improve the variable response, xylanase activity. The condition that promoted the highest production, 13.02 UmL 1 (141% higher than the initial condition), was 20 g-L-1 wheat bran, 2.5 g L-1 yeast extract, 3 g L-1 K2HPO4, and pH 7. Thus, industrial byproducts with a high content of xylan can be used as a culture medium to produce xylanase enzymes with a Talaromyces strain through an economical and sustainable approach. (AU) | |
| Processo FAPESP: | 14/01580-3 - Processo biotecnológico visando o desenvolvimento de novos colorantes naturais microbianos para aplicação industrial |
| Beneficiário: | Valéria de Carvalho Santos Ebinuma |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 19/15493-9 - Desenvolvimento de hidrogéis eletrocondutivos baseados em líquidos iônicos |
| Beneficiário: | Valéria de Carvalho Santos Ebinuma |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/06908-8 - Desenvolvimento de nanomateriais sustentáveis para a purificação de fármacos antileucêmicos |
| Beneficiário: | Valéria de Carvalho Santos Ebinuma |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/06686-8 - Processo biotecnológico visando o desenvolvimento de colorantes naturais microbianos para aplicação industrial: fase II |
| Beneficiário: | Valéria de Carvalho Santos Ebinuma |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 20/15513-7 - Scale up da produção de L-asparaginase empregando microrganismo geneticamente modificado |
| Beneficiário: | Heitor Buzetti Simões Bento |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |