| Texto completo | |
| Autor(es): |
Bernardes, Carolina P.
;
Santos, Neife A. G.
;
Costa, Tassia R.
;
Menaldo, Danilo L.
;
Sisti, Flavia M.
;
Amstalden, Martin K.
;
Ribeiro, Diego L.
;
Antunes, Lusania M. G.
;
Sampaio, Suely V.
;
Santos, Antonio C.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS; v. 29, n. 3, p. 16-pg., 2023-04-03. |
| Resumo | |
We have recently reported the neurotrophic and neuroprotective effects of the snake-venom-based synthetic tripeptide BTX-I in PC12 cells treated with acrolein. In the present study, this peptide was chemically modified to increase its neurotrophic/neuroprotective activity. Esterification (ethyl or methyl), PEGylation and amidation were introduced at the C-terminus; acetylation was introduced at the N-terminus. The modified peptides protected PC12 cells from the decrease in viability and neuritogenesis induced by acrolein; however, only the ethyl-esterified peptide (named BTX-II) significantly increased neuritogenesis in comparison with the original peptide BTX-I. Moreover, BTX-II increased the expression of proteins related to (i) axonal/synaptic plasticity (synapsin I, beta-III-Tubulin), and (ii) energy metabolism (AMPK-alpha and SIRT I) in PC12 cells treated with acrolein. In addition, BTX-II upregulated the expression of genes that encode apolipoprotein E (ApoE) and Mitogen-Activated Protein Kinase 3 (Mapk3), which are associated with cognitive improvements in animal models of Alzheimer's disease (AD). In conclusion, ethyl-esterification at the C-terminus of the snake-venom-based tripeptide [Glu-Val-Trp] improved the neurotrophic and neuroprotective potential in relation to the original tripeptide. (AU) | |
| Processo FAPESP: | 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular |
| Beneficiário: | Suely Vilela |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |