Oxygen uptake and transfer rates throughout production of recombinant baculovirus and rabies virus-like particles

Autor(es)

Oliveira Guardalini, Luis Giovani ; da Silva Cavalcante, Paulo Eduardo ; Leme, Jaci ; de Mello, Renata Gois ; Bernardino, Thaissa Consoni ; Astray, Renato Mancini ; Tonso, Aldo ; Calil Jorge, Soraia Attie ; Fernandez Nunez, Eutimio Gustavo

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 124, p. 12-pg., 2022-12-06.

Resumo

The technologies used in rabies vaccines manufacturing for human use are based on the inactivated virus platform. An alternative to traditional vaccines is virus-like particles (VLPs). This work aimed to characterize the oxygen uptake and transfer rate parameters throughout recombinant baculovirus (rBV) and rabies VLPs production using Sf9 cells in stirred tank bioreactor (STB) for a better bioprocess understanding and scalability. Four runs in a bench STB were performed: cell culture without infection; cells infected singly with rBV bearing rabies virus glycoprotein (rBVG, multiplicity of infection, MOI=0.1 pfu/cell) and matrix protein (rBVM, MOI=0.1 pfu/ cell), and coinfected with BVG and BVM at MOI of 3 and 2 pfu/cell, respectively. The specific oxygen uptake rate (q(O2)) and volumetric oxygen transfer coefficient (k(L)(a)) were monitored throughout the reactions, as well as viable cell concentration, viability, rBV titers, and protein concentration. According to the results herein, the aeration and agitation systems in a bioreactor at a higher scale could be designed using the criterium for scale-up of constant kLa, without oxygen facilities. Besides, rabies VLPs volumetric yield of 2.8 mg/L with a typical size (55-68 nm) was obtained. These findings suggest a promising bioprocess for rabies VLPs at a commercial scale. (AU)

• Processo FAPESP: 18/10538-1 - Caracterização de antígenos recombinantes e da resposta imune para um novo candidato vacinal contra a raiva
• Beneficiário: Renato Mancini Astray
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/22780-6 - Produção e caracterização de VLPs de Arbovírus produzidos em sistemas de células de inseto.
• Beneficiário: Soraia Attie Calil Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular