Detection of DENV-2 and ZIKV coinfection in southeastern Brazil by serum and urine testing

Autor(es)

da Conceicao, Pamela Joyce Previdelli ; de Carvalho, Lucas Rodrigues ; de Godoy, Bianca Lara Venancio ; Nogueira, Mauricio Lacerda ; Terzian, Ana Carolina Bernardes ; de Godoy, Moacir Fernandes ; Calmon, Marilia Freitas ; Bittar, Cintia ; Rahal, Paula

• Tipo de documento: Artigo Científico
• Fonte: MEDICAL MICROBIOLOGY AND IMMUNOLOGY; v. 212, n. 3, p. 9-pg., 2023-04-07.

Resumo

PurposeAedes aegypti mosquito-borne diseases have a significant impact on public health in Brazil. In this study, we investigated the presence of the Zika virus (ZIKV) and dengue virus (DENV) in serum and urine samples from symptomatic participants who attended an Emergency Care Unit located in a city in the northwestern region of Sao Paulo between February 2018 and April 2019.MethodsSerum and urine samples were collected from participants suspected of having arbovirus infection. After the extraction of viral RNA, viral detection was performed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) (One-Step RT-qPCR).ResultsA total of 305 participants participated in this study. A total of 283 blood and 270 urine samples were collected. Of 305 patients, 36.4% (111/305) were positive for ZIKV, 43.3% (132/305) for DENV2, and 0.3% (1/305) for DENV1. Coinfection with ZIKV/DENV2 was observed in 13.1% of participants. If only serum samples were used, ZIKV detection would have decreased to 23.3% (71/305). Of all the participants included in the study, only one was suspected of having ZIKV infection based on clinical diagnosis, and the remaining participants were suspected of having DENV.ConclusionBy testing serum and urine samples, we increased the detection of both viruses and detected considerable levels of ZIKV and DENV-2 coinfection when compared to other studies. Additionally, we detected an unnoticed ZIKV outbreak in the city. These findings highlight the importance of the molecular diagnosis of arboviruses to aid public health surveillance and management strategies. (AU)

• Processo FAPESP: 14/22198-0 - Potencial terapêutico de fármacos flavonóidicos na infecção com o vírus da hepatite C
• Beneficiário: Paula Rahal
• Modalidade de apoio: Auxílio à Pesquisa - Regular