| Texto completo | |
| Autor(es): |
da Silva Feltrin, Felipe
;
D'Angelo, Natalia Aimee
;
de Oliveira Guarnieri, Joao Paulo
;
Lopes, Andre Moreni
;
Lancellotti, Marcelo
;
Lona, Liliane Maria Ferrareso
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 22, p. 14-pg., 2023-05-23. |
| Resumo | |
Curcumin (CUR) is one natural bioactive compound acknowledgedfordiverse therapeutic activities, but its use is hindered by its poorbioavailability, fast metabolism, and susceptibility to pH variationsand light exposure. Thus, the encapsulation in poly-(lactic-co-glycolic acid), or PLGA, has been successfully used toprotect and enhance CUR absorption in the organism, making CUR-loadedPLGA nanoparticles (NPs) promising drug delivery systems. However,few studies have focused beyond CUR bioavailability, on the environmentalvariables involved in the encapsulation process, and whether theycould help obtain NPs of superior performance. Our study evaluatedpH (3.0 or 7.0), temperature (15 or 35 degrees C), light exposure, andinert atmosphere (N-2) incidence in the encapsulation ofCUR. The best outcome was at pH 3.0, 15 degrees C, without light incidence,and without N-2 usage. This best nanoformulation showedNP size, zeta potential, and encapsulation efficiency (EE) of 297nm, -21 mV, and 72%, respectively. Moreover, the CUR in vitro release at pH values 5.5 and 7.4 suggested differentpotential applications for these NPs, one of which was demonstratedby the effective inhibition of multiple bacteria (i.e., Gram-negative, Gram-positive, and multi-resistant) in the minimalinhibition concentration assay. Besides, statistical analyses confirmeda significant impact of temperature on the NP size; in addition, temperature,light, and N-2 affected the EE of CUR. Thus, the selectionand control of process variables resulted in higher CUR encapsulationand customizable outcomes, ultimately enabling more economical processesand providing future scale-up guidelines. (AU) | |
| Processo FAPESP: | 18/12831-8 - Síntese in situ de polímeros biodegradáveis usando como carga celulose nanocristalina, microfibrilada e lignina sem funcionalização |
| Beneficiário: | Liliane Maria Ferrareso Lona |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/09341-1 - Materiais poliméricos funcionais alternativos com potencial aplicação no auxílio do tratamento e da poliferação de doenças |
| Beneficiário: | Liliane Maria Ferrareso Lona |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/03727-2 - Desenvolvimento de polimerossomos visando a co-encapsulação de fármacos para aplicação na terapia do câncer |
| Beneficiário: | Natalia Aimee D'Angelo |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 18/10799-0 - Terapia combinatória utilizando polimerossomos decorados com transferrina e incorporados em hidrogéis de quitosana como smart drug delivery systems para células tumorais de melanoma |
| Beneficiário: | André Moreni Lopes |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |
| Processo FAPESP: | 17/10789-1 - Terapia combinatória utilizando polimerossomos decorados com transferrina e incorporados em hidrogéis de quitosana como smart drug delivery systems para células tumorais de melanoma |
| Beneficiário: | André Moreni Lopes |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |