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Entree


Glycomic profiling identifies key-structural differences in three arabinoxylan fractions from sugarcane culms

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Autor(es):
Garbelotti, Carolina Victal ; Grandis, Adriana ; Crevelin, Eduardo ; Buckeridge, Marcos Silveira ; de Moraes, Luiz Alberto Beraldo ; Ward, Richard John
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Carbohydrate Polymers; v. 310, p. 13-pg., 2023-02-24.
Resumo

Sugarcane is an important food and bioenergy crop, and although the residual biomass is potentially available for biorefinery and biofuels production the complex plant cell wall matrix requires pretreatment prior to enzymatic hydrolysis. Arabinoxylans require multiple enzymes for xylose backbone and saccharide side-branch hydrolysis to release xylooligosaccharides and pentoses. The effect of arabinoxylan structure on xylooligosaccharide release by combinations of up to five xylanolytic enzymes was studied using three arabinoxylan fractions extracted from sugarcane culms by sodium chlorite, DMSO and alkaline treatments. Reducing sugar release and LC-MS detection with chemometric analysis identified different xylooligosaccharide profiles between extracts following enzyme treatments. The position and degree of side-branch decorations are determinants of enzyme activity and xylooligosaccharide diversity with the alkaline and post-sodium chlorite extracts as the most accessible and most recalcitrant, respectively, indicating acetyl substituents as a major recalcitrance factor. The complex xylooli-gosaccharide profile with the DMSO extract suggests regions with different levels of branching. Chemometric analysis identified GH10 xylanase hydrolysis products that act as substrates for other enzymes, such as alpha-glucuronidase. The strategy reported here can identify specific enzyme combinations to overcome barriers for biomass processing such as pretreatment selection, recalcitrance to enzyme digestion and optimization of reducing sugar release. (AU)

Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
Beneficiário:Marcos Silveira Buckeridge
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/24139-6 - Desenho e Caracterização de Enzimas Xilanolíticas Multifuncionais
Beneficiário:Richard John Ward
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 19/13936-0 - O papel do xiloglucano na determinação da arquitetura da parede celular de cana de açúcar
Beneficiário:Adriana Grandis
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado