Profile of MicroRNAs Associated with Death Due to Disease Progression in Metastatic Papillary Thyroid Carcinoma Patients

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Leite, Ana Kober ; Saito, Kelly Cristina ; Theodoro, Therese Rachell ; Pasini, Fatima Solange ; Camilo, Luana Perrone ; Rossetti, Carlos Augusto ; Cavalheiro, Beatriz Godoi ; Alves, Venancio Avancini Ferreira ; Kowalski, Luiz Paulo ; Pinhal, Maria Aparecida Silva ; Kimura, Edna Teruko ; Matos, Leandro Luongo

• Tipo de documento: Artigo Científico
• Fonte: CANCERS; v. 15, n. 3, p. 12-pg., 2023-02-01.

Resumo

Papillary thyroid cancer is not an aggressive cancer, even when metastasis are present; therefore, treatment has been downgraded in recent years. However rare, mortality exists and finding the factors associated with mortality is essential and has not yet been fully accomplished; the answer probably lies in molecular factors. This study aimed to find MicroRNAs related to death in metastatic patients and found that patients who died due to progression of PTC had higher expression levels of miR-101-3p, miR-17-5p, and miR-191-5p compared with those of patients with stable metastatic disease. These findings are new in the literature and may open new doors in differentiating the patients who need more aggressive treatment from those who do not. Papillary thyroid carcinoma (PTC) is the most common neoplasm of the endocrine system and has an excellent long-term prognosis, with low rates of distant metastatic disease. Although infrequent, there are cases of deaths directly related to PTC, especially in patients with metastatic disease, and the factors that could be associated with this unfavorable outcome remain a major challenge in clinical practice. Recently, research into genetic factors associated with PTC has gained ground, especially mutations in the TERT promoter and BRAF gene. However, the role of microRNAs remains poorly studied, especially in those patients who have an unfavorable outcome at follow-up. This paper aims to evaluate molecular markers related to the different pathological processes of PTC, as well as the histological characteristics of the neoplasm, and to compare this profile with prognosis and death from the disease using an analysis of patients treated for metastatic disease in a single tertiary cancer center. Evaluation of microRNA expression in paraffin-embedded tumor specimens was carried out by quantitative PCR using the TaqMan((R)) Low Density Array (TLDA) system. Metastatic patients who died from progression of PTC had higher expressions of miR-101-3p, miR-17-5p, and miR-191-5p when compared to patients with stable metastatic disease. These findings are of great importance but should be considered as preliminary because of the small sample. (AU)

• Processo FAPESP: 20/12459-1 - Análise de marcadores imunoistoquímicos relacionados a metástase e a óbito no carcinoma papilífero de tireoide
• Beneficiário: Luana Perrone Camilo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/25116-8 - Modulação de microRNAs e sua rede regulatória de alvos com potencial terapêutico no câncer de tiroide: aplicação da edição gênica com CRISPR/Cas9
• Beneficiário: Edna Teruko Kimura
• Modalidade de apoio: Auxílio à Pesquisa - Regular