Emerging role of microglia and astrocyte in the affective-motivational response induced by a rat model of persistent orofacial pain

Autor(es)

Nascimento, Glauce Crivelaro ; Lucas, Guilherme ; Andrade Leite-Panissi, Christie Ramos

• Tipo de documento: Artigo Científico
• Fonte: Brain Research Bulletin; v. 195, p. 13-pg., 2023-02-20.

Resumo

Few studies are approaching the neural basis underlying the aggregation of emotional disorders in orofacial pain despite the stress, depression, and anxiety are some of the most commonly reported risk factors. Using a persistent orofacial pain rat model induced by complete Freund's adjuvant (CFA) injection into the temporomandibular joint, we have investigated the plasticity astrocytes and microglia key brain regions for the affectiveemotional component of pain. We measured the expression and morphologic pattern of reactivation of glial fibrillary acidic protein (GFAP, astrocyte marker) and Iba-1 (microglial marker) by western blotting and immunohistochemistry analysis. The results showed no alterations on motor activity during inflammatory pain, indicating an exclusive effect of nociceptive behavior on the plasticity of limbic regions. CFA-induced temporomandibular inflammation changed GFAP and Iba-1 expression in distinct regions related to emotional behavior in a time-dependent manner. A significant increase in GFAP and Iba-1 expression was observed in the central nucleus of the amygdala, hippocampus and periaqueductal grey matter from day 3 to day 10 post-CFA injection. Moreover, a positive correlation between GFAP and Iba-1 upregulation and an increased mechanical hypersensitivity was observed. Conversely, no change on GFAP and Iba-1 expression was observed in the hypothalamus and colliculus during orofacial inflammatory pain. Our data suggest an important role for glial cells in the affective-motivational dimension of orofacial pain beyond their well-explored role in the traditional nociceptive transmission circuits. (AU)

• Processo FAPESP: 15/03053-3 - Avaliação de músculo pterigóideo medial e estruturas encefálicas relacionadas à nocicepção orofacial e comportamento emocional em modelo animal de estresse crônico e hipofunção mastigatória
• Beneficiário: Glauce Crivelaro Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/07718-9 - Análise comportamental de ratos portadores de inflamação persistente da articulação temporomandibular: correlação com a quantificação de metaloproteinases em estruturas do sistema cerebral aversivo
• Beneficiário: Glauce Crivelaro Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado