Effect of CO2 laser combined with AmF/NaF/SnCl2 solution on the prevention of human and bovine enamel erosion

Autor(es)

Engel, Yael ; da Silva, Camila Vieira ; Ramos-Oliveira, Thayanne Monteiro ; Mantilla, Tais Fonseca ; Tavares, Juliane de Paula ; de Freitas, Patricia Moreira

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 36, p. 11-pg., 2022-01-01.

Resumo

This in vitro study evaluated the potential of CO2 laser (10.6 mu m) combined with a stannous/fluoride-containing solution for preventing erosion in human/bovine enamel. Forty-eight samples of each substrate were randomly allocated to four groups (n = 12): W - distilled water; E - AmF/NaF/SnCl2 solution; L - CO2 laser; and LE - CO2 laser+AmF/NaF/SnCl2 solution. After surface treatments, samples were submitted to a 5-day erosive challenge, alternating immersions in 0.5% citric acid (2 minutes, 6x/day) and in artificial saliva. Optical profilometry (mu m) and scanning electron microscopy (SEM) were used to determine surface loss and surface morphology, respectively. Data were statistically analyzed by two-way ANOVA and Tukey's tests (p < 0.05). For human enamel, tissue loss was lower in group L (12.37 +/- 4.46) than in group W (16.45 +/- 2.76), and higher than in the groups treated with AmF/NaF/SnCl2 solution (E-5.44 +/- 2.37; LE-5.55 +/- 2.31). In group L, SEM images reveled a disorganized surface but fewer projections than in group W and LE showed fewer irregularities than W, E, and L. For bovine enamel, tissue loss in group L (13.90 +/- 3.50) did not differ from that in group W (14.10 +/- 2.98), and was higher than losses in groups E (5.70 +/- 2.12) and LE (8.12 +/- 2.56), which were statistically similar to each other. Groups W and L had similar aspects of demineralization, whereas groups E and LE showed homogenous surfaces. Surface-treated samples had no changes in their surfaces. CO2 laser was able to slightly prevent surface loss only on human enamel surface, but did not enhance the AmF/NaF/SnCl2 effect on the prevention of enamel erosion. (AU)

• Processo FAPESP: 16/08101-9 - Avaliação in vitro do potencial do laser de CO2 (10,6 µm), associado ou não ao fluoreto estanhoso, em prevenir lesão de erosão em esmalte dental humano e bovino
• Beneficiário: Yael Engel
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica