Interactions with HSA, anticancer and antiallergic activity of binuclear mu-oxo bridged ruthenium acetate compounds

Autor(es)

Cacita, Natacha ; Silva, Amanda Batista ; dos Santos, Nicolle Azevedo Portela ; Ramos, Loyanne Carla Barbosa ; Del Lama, Maria Perpetua Freire de Moraes ; Naal, Rose Mary Zumstein Georgetto ; Nikolaou, Sofia

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRYSELECT; v. 8, n. 13, p. 10-pg., 2023-04-05.

Resumo

The mu-oxo bridged ruthenium acetate 1 [Ru2O(CH3COO)(2)(5-CH3-1,10-phen)(2)(py)(2)](PF6)(2) (5-CH3-1,10-phen=5-methyl-1,10-phenanthroline; py=pyridine) is presented. Its electronic and infrared spectra, as well as its cyclic voltammograms are all consistent with the proposed structure. Regarding biological properties, we collected data for 1 and its analog [Ru2O(CH3COO)(2)(1,10-phen)(2)(py)(2)](PF6)(2) (2) to infer the role of phenantroline methylation. The HSA fluorescence is quenched by 1 and the presence of variable concentrations of it did not affect the tau(1/2) values for the HSA excited state lifetime (mean tau(1/2) values=3.56, 3.46, and 3.32 ns at 298, 304, and 310 K respectively). Circular dichroism showed that the HSA alpha-helix content decreased only 5 % upon interaction with 1, which formed a ground-state adduct with HSA, not changing the protein structure to a significant extent. Interaction between 1 and DNA was weak; Benesi-Hildebrand constants were in the order of 10(2) M-1. We probed the allergenic potential/antiallergic activity of 1, observing that 200 mu M inhibited mast cell degranulation by about 80 %, while cell viability remained unaltered throughout the measurement (2 h). Therefore, 1 has antiallergic potential and is not an allergen. Regarding its anticancer activity, at all the employed concentrations, 1 was more cytotoxic than 2 against B16F10 murine melanoma cancer cells. At 25 mu M, 1 reduced cell viability to less than 14 %, while 2 reduced cell viability to only 78 %. (AU)

• Processo FAPESP: 22/03478-8 - Reatividade de compostos polinucleares de rutênio com potencial aplicação biológica
• Beneficiário: Sofia Nikolaou
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/02802-3 - Estudo de reatividade redox e liberação de óxido nítrico e monóxido de carbono em carboxilatos trinucleares de rutênio
• Beneficiário: Amanda Batista Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/18060-3 - Uso de estruturas supramoleculares inorgânicas com vistas ao desenvolvimento de moléculas funcionais e liberação controlada de espécies bioativas
• Beneficiário: Sofia Nikolaou
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/14170-1 - Estudo de potenciais liberadores de óxido nítrico baseado em complexos de rutênio e ligantes azanaftalenos
• Beneficiário: Nicolle Azevedo Portela dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica