Inflammatory stimulus worsens the effects of UV-A exposure on J774 cells

Autor(es)

Chiarelli-Neto, Orlando ; Garcez, Michelle Lima ; Pavani, Christiane ; Martins, Waleska ; Castro, Fernanda Cristina de Abreu Quintela ; Ambrosio, Roberta Passamani ; Meotti, Flavia Carla ; Baptista, Mauricio S.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 239, p. 9-pg., 2023-01-10.

Resumo

UV-A radiation affects skin homeostasis by promoting oxidative distress. Endogenous photosensitizers in the dermis and epidermis of human skin absorb UV-A radiation forming excited states (singlet and triplet) and reactive oxygen species (ROS) producing oxidized compounds that trigger biological responses. The activation of NF-kB induces the expression of pro-inflammatory cytokines and can intensify the generation of ROS. However, there is no studies evaluating the cross talks between inflammatory stimulus and UV-A exposure on the levels of redox misbalance and inflammation. In here, we evaluated the effects of UV-A exposure on J774 macrophage cells previously challenged with LPS in terms of oxidative distress, release of pro-inflammatory cytokines, and activation of regulated cell death pathways. Our results showed that LPS potentiates the dose-dependent UV-A -induced oxidative distress and cytokine release, in addition to amplifying the regulated (autophagy and apoptosis) and non-regulated (necrosis) mechanisms of cell death, indicating that a previous inflammatory stimulus potentiates UV-A-induced cell damage. We discuss these results in terms of the current-available skin care strategies. (AU)

• Processo FAPESP: 18/22922-0 - Modulação da autofagia por estresse bioquímico e fotoquímico: implicações terapêuticas
• Beneficiário: Waleska Kerllen Martins Gardesani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 18/23257-0 - Modulação de danos paralelos bioquímicos e fotoquímicos: implicações na autofagia pró-sobrevida
• Beneficiário: Maryana do Nascimento da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica