Characterization of Paracoccidioides brasiliensis COX9, COX12, and COX16 respiratory genes

Autor(es)

Bandeira, Simone C. B. ; Nobrega, Marina P.

• Tipo de documento: Artigo Científico
• Fonte: MYCOLOGICAL RESEARCH; v. 112, p. 7-pg., 2008-12-01.

Resumo

Paracoccidioides brasiliensis is a thermo-dimorphic fungus that is the causative agent of paracoccidioidomyicosis (PCM), a human systemic granulomatous mycosis found in Latin America. Dimorphic transition from mycelium to yeast is required for establishing pathogenicity. Dimorphism is marked by changes in mitochondrial physiology, including modulation of respiration rate. In this work, we present the identification of three P. brasiliensis nuclear genes PbCOX9, PbCOX12, and PbCOX16 that code for structural sub-units and a putative assembly facilitator (PbCOX16) of the mitochondrial cytochrome c oxidase (COX), the terminal enzyme complex of the respiratory chain. We measured their expression pattern during the dimorphic transition from mycelium to yeast and back by real-time reverse transcription quantitative polymerase chain reaction (real-time RT-qPCR). Our results show that messages from these genes increase during the mycelium to yeast transition and decrease during the opposite conversion. This result supports active mitochondrial participation in the transition. Heterologous complementation of the corresponding Saccharomyces cerevisiae null mutant with the PbCOX9 gene was successfully obtained. (C) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/07324-7 - Sequenciamento de clones genomicos completos de paracoccidioides brasiliensis.
• Beneficiário: Marina Pasetto Nobrega
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/08711-9 - Caracterização molecular de genes envolvidos no processo de patogenicidade/virulência no fungo patogênico humano Paracoccidioides brasiliensis
• Beneficiário: Gustavo Henrique Goldman
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 04/05559-7 - Identificacao funcional e estrutura genomica de 20 genes nucleares associados a atividade da citocromo oxidase em paracoccidioides brasiliensis.
• Beneficiário: Simone Cristina Borges Bandeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado