| Texto completo | |
| Autor(es): |
dos Reis, Caio Vinicius
;
Bernardes, Amanda
;
Polikarpov, Igor
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 69, p. 4-pg., 2013-05-01. |
| Resumo | |
Xylose isomerase (EC 5.3.1.5) is a key enzyme in xylose metabolism which is industrially important for the transformation of glucose and xylose into fructose and xylulose, respectively. The Bifidobacterium adolescentis xylA gene (NC_008618.1) encoding xylose isomerase (XI) was cloned and the enzyme was overexpressed in Escherichia coli. Purified recombinant XI was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as the precipitating agent. A complete native data set was collected to 1.7 angstrom resolution using a synchrotron-radiation source. The crystals belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 88.78, b = 123.98, c = 78.63 angstrom. (AU) | |
| Processo FAPESP: | 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |