| Texto completo | |
| Autor(es): Mostrar menos - |
Sampaio, Suely C. F.
;
Moreira, Fabiana C.
;
Liberatore, Ana M. A.
;
Vieira, Monica A. M.
;
Knobl, Terezinha
;
Romao, Fabiano T.
;
Hernandes, Rodrigo T.
;
Ferreira, Claudete S. A.
;
Ferreira, Antonio P.
;
Felipe-Silva, Aloisio
;
Sinigaglia-Coimbra, Rita
;
Koh, Ivan H. J.
;
Gomes, Tania A. T.
Número total de Autores: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMED RESEARCH INTERNATIONAL; v. 2014, p. 9-pg., 2014-01-01. |
| Resumo | |
Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites. (AU) | |
| Processo FAPESP: | 08/53812-4 - Invasão: um mecanismo potencial de virulência em Escherichia coli enteropatogênica atípica? |
| Beneficiário: | Tânia Aparecida Tardelli Gomes do Amaral |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/12664-5 - Explorando as interações de Escherichia coli enteropatogênica com células intestinais in vitro e in vivo |
| Beneficiário: | Tânia Aparecida Tardelli Gomes do Amaral |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |