Rational development of a novel TFPI-like inhibitor from Amblyomma cajennense tick

Autor(es)

Morais, Katia L. P. ; Mesquita Pasqualoto, Kerly Fernanda ; Fernandes Pacheco, Mario Thiego ; Berra, Carolina Maria ; Alvarez-Flores, Miryam Paola ; Chudzinski-Tavassi, Ana Marisa

• Tipo de documento: Artigo Científico
• Fonte: Toxin Reviews; v. 33, n. 1-2, p. 5-pg., 2014-03-01.

Resumo

Hematophagous organisms have been described to possess specific blood coagulation inhibitors with different structures and novel mechanism of action. A factor Xa inhibitor was identified through the salivary glands transcriptome from the adult Amblyomma cajennense tick. The recombinant form was expressed in Escherichia coli and named Amblyomin-X. The recombinant protein not only inhibits factor X, but is also able to inhibit the activation of factor X by the extrinsic tenase complex. It seems to activate the apoptosis pathway through proteasome inhibition and endoplasmic reticulum-stress. It promotes regression and reduction of lung metastasis in murine melanoma tumor, and does not affect normal cells, emphasizing its selectivity of action. (AU)

• Processo FAPESP: 11/05969-4 - Estudo do papel da dineína no mecanismo de ação antitumoral do Amblyomin-X em células de melanoma e adenocarcinoma de pâncreas
• Beneficiário: Mario Thiego Fernandes Pacheco
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/07958-7 - Avaliação do mecanismo de ação pró-apoptótica do amblyomin-x
• Beneficiário: Kátia Luciano Pereira Morais
• Modalidade de apoio: Bolsas no Brasil - Doutorado