Investigation of DMSO-Induced Conformational Transitions in Human Serum Albumin Using Two-Dimensional Raman Optical Activity Spectroscopy

Autor(es)

Batista, Andrea N. L. ; Batista, Joao M., Jr. ; Ashton, Lorna ; Bolzani, Vanderlan S. ; Furlan, Maysa ; Blanch, Ewan W.

• Tipo de documento: Artigo Científico
• Fonte: CHIRALITY; v. 26, n. 9, p. 5-pg., 2014-09-01.

Resumo

Recent Raman and Raman optical activity (ROA) results have demonstrated that dimethyl sulfoxide (DMSO) induces the selective conversion of alpha-helix motifs into the poly(L-proline) II (PPII) helix conformation in an array of proteins, while beta-sheets remain mostly unaffected. Human serum albumin (HSA), a highly alpha-helical protein, underwent the most dramatic changes and, therefore, was selected as a model for further investigations into the mechanism of this conformational change. Herein we report the use of two-dimensional ROA correlation analysis applying synchronous, autocorrelation, and moving windows approaches in order to understand the conformational transitions in HSA as a function of DMSO concentration. Our results indicate that the destabilization of native alpha-helix starts at DMSO concentrations as little as 20% in water (v/v), with the transition to PPII helix being complete at similar to 80% DMSO. These results clearly indicate that any protein preparation containing relatively low concentrations of DMSO should consider possible disruptions in alpha-helical domains. (C) 2014 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 12/13739-1 - Atividade óptica vibracional no estudo da quiralidade molecular
• Beneficiário: João Marcos Batista Junior
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/16484-4 - Atividade óptica Raman: uma ferramenta inovadora para análise estrutural de proteínas
• Beneficiário: Andrea Nastri de Luca Batista
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado