| Texto completo | |
| Autor(es): |
Batista, Andrea N. L.
;
Batista, Joao M., Jr.
;
Ashton, Lorna
;
Bolzani, Vanderlan S.
;
Furlan, Maysa
;
Blanch, Ewan W.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHIRALITY; v. 26, n. 9, p. 5-pg., 2014-09-01. |
| Resumo | |
Recent Raman and Raman optical activity (ROA) results have demonstrated that dimethyl sulfoxide (DMSO) induces the selective conversion of alpha-helix motifs into the poly(L-proline) II (PPII) helix conformation in an array of proteins, while beta-sheets remain mostly unaffected. Human serum albumin (HSA), a highly alpha-helical protein, underwent the most dramatic changes and, therefore, was selected as a model for further investigations into the mechanism of this conformational change. Herein we report the use of two-dimensional ROA correlation analysis applying synchronous, autocorrelation, and moving windows approaches in order to understand the conformational transitions in HSA as a function of DMSO concentration. Our results indicate that the destabilization of native alpha-helix starts at DMSO concentrations as little as 20% in water (v/v), with the transition to PPII helix being complete at similar to 80% DMSO. These results clearly indicate that any protein preparation containing relatively low concentrations of DMSO should consider possible disruptions in alpha-helical domains. (C) 2014 Wiley Periodicals, Inc. (AU) | |
| Processo FAPESP: | 12/13739-1 - Atividade óptica vibracional no estudo da quiralidade molecular |
| Beneficiário: | João Marcos Batista Junior |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos |
| Beneficiário: | Glaucius Oliva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 12/16484-4 - Atividade óptica Raman: uma ferramenta inovadora para análise estrutural de proteínas |
| Beneficiário: | Andrea Nastri de Luca Batista |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |